Cloning and expression of the gene encoding (R)-specific carbonyl reductase from CCTCC M203011

Abstract

The gene which encodes (R)-specific carbonyl reductase (rCR) from Candida parapsilosis CCTCC M203011 was cloned, sequenced and compared with genes from the GenBank. The results indicated that rCR gene was 1011 bp, encoding a protein of 336 amino acids with a molecular weight of 35.9 kDa, and its nucleotide sequence showed 99% similarity to those of other members of the alcohol dehydrogenase superfamily. The rCR gene could express in recombinant strain Escherichia coli JM109, and the expression plasmid could produce (R)-1-pheny-1,2-ethanediol (100% e.e., 80.14% yield) from ?-hydroxyacetophenone without any additive to regenerate NAD⁺ from NADH.

Keywords

carbonyl reductase, expression, asymmetric reduction, (R)-1-phenyl-1, 2-ethanediol