DNA duplex membrane effect for the electrochemical detection of single-base DNA mutations

Luo Chunxiong1, Mao Yongdong1, Ouyang Qi2

PDF(505 KB)
PDF(505 KB)
Front. Biol. ›› 2006, Vol. 1 ›› Issue (2) : 203-207. DOI: 10.1007/s11515-006-0019-6

DNA duplex membrane effect for the electrochemical detection of single-base DNA mutations

  • Luo Chunxiong1, Mao Yongdong1, Ouyang Qi2
Author information +
History +

Abstract

Here we report a new method to detect DNA point mutations. The method is based on the formation and deformation of double-stranded DNA (dsDNA) membranes on a gold surface. It can encage reporter molecules between the gold surface and the double-stranded DNA or keep them away from the gold surface. In these systems, Fe(CN)63- was used as the reporter. As the temperature increases, a sharp electrochemical signal change in the melting curve of wild-type dsDNA appears. At a special temperature, the method gives 100:1 selectivity for the perfect complement and single base mutation target. Thus, the system provides a simple and sensitive method to detect DNA point mutations without labeling targets.

Cite this article

Download citation ▾
Luo Chunxiong, Mao Yongdong, Ouyang Qi. DNA duplex membrane effect for the electrochemical detection of single-base DNA mutations. Front. Biol., 2006, 1(2): 203‒207 https://doi.org/10.1007/s11515-006-0019-6
PDF(505 KB)

Accesses

Citations

Detail

Sections
Recommended

/