DNA duplex membrane effect for the electrochemical detection of single-base DNA mutations
Luo Chunxiong1, Mao Yongdong1, Ouyang Qi2
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1.Laboratory for Biophysics and Biotechnology, Department of Physics, Beijing 100871, China; 2.Laboratory for Biophysics and Biotechnology, Department of Physics, Beijing 100871, China; Center for Theoretical Biology, Peking University, Beijing 100871, China;
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Published
05 Jun 2006
Issue Date
05 Jun 2006
Abstract
Here we report a new method to detect DNA point mutations. The method is based on the formation and deformation of double-stranded DNA (dsDNA) membranes on a gold surface. It can encage reporter molecules between the gold surface and the double-stranded DNA or keep them away from the gold surface. In these systems, Fe(CN)63- was used as the reporter. As the temperature increases, a sharp electrochemical signal change in the melting curve of wild-type dsDNA appears. At a special temperature, the method gives 100:1 selectivity for the perfect complement and single base mutation target. Thus, the system provides a simple and sensitive method to detect DNA point mutations without labeling targets.
Luo Chunxiong, Mao Yongdong, Ouyang Qi.
DNA duplex membrane effect for the electrochemical detection of single-base DNA mutations. Front. Biol., 2006, 1(2): 203‒207 https://doi.org/10.1007/s11515-006-0019-6
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