Frontiers of Forestry in China >

2009 , Vol. 4 >Issue 4: 489 - 493

DOI: https://doi.org/10.1007/s11461-009-0069-4

Research articles

Phylogenetic relationship of genus Lespedeza by ITS sequence data

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  • 1.Forestry College of Guizhou University, Guiyang 550225, China; 2.College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China; 3.South China Agricultural University, Guangzhou 510642, China;

Published date: 05 Dec 2009

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Abstract

ITS sequences of ten kinds of plants of Lespedeza and an out group were obtained by primer design PCR, sequencing and cluster analysis. The results show that ITS1 section length was 228―243 bp, 5.8S sequence length was 165 bp which was very conservative; ITS2 section lengths varied from 215 to 220 bp and the conservative sites occupied 88.1%. Phylogenetic analysis of ITS sequences using PAUP 4.0 software and their genetic relationship were discussed.

Key words： Lespedeza Michx; internal transcribed spacer (ITS); phylogeny

Cite this article

Xiurong WANG, Yang ZHAO, Ruiqi PIAN, Xiaoyang CHEN, . Phylogenetic relationship of genus Lespedeza by ITS sequence data[J]. Frontiers of Forestry in China, 2009 , 4(4) : 489 -493 . DOI: 10.1007/s11461-009-0069-4

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