Role of cell wall degrading enzymes in the interaction of poplar and Melampsora larici-populina Kleb.

Abstract

The activity of cell wall-degrading enzymes, produced in poplar cultivars infected Melampsora larici-populina Kleb., was studied. The results show that PMG, PMTE, C_X and β-glucosidase played roles during the infection. After inoculation, the activity of PMG in both susceptible and resistant cultivars had two peak values in 2 dpi and 5 dpi. The activities of PMTE and β-glucosidase had a peak value in 3 dpi, and C_X in 2 dpi. Among these cell wall-degrading enzymes, the activities of PMG and PMTE were higher and the activities of C_X and β-glucosidase were relatively lower. The activities of these cell wall-degrading enzymes were significantly higher in susceptible cultivars than those in resistant cultivars. All these demonstrated that these cell wall-degrading enzymes played certain roles in the infection of M. larici-populina.

Key words： Melampsora larici-populina; cell wall-degrading enzymes; poplar

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 30471394) and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education.

References

1 Cao Z M, Li Z Q, Hu J J (1998). Physiological specialization in Melampsora larici-populinaKleb. in the Qinling Mountains. J Northwest For Coll,13(1): 53–57 (in Chinese)

2 Chen X J, Zhang H, Xu J Y, Tong Y H, Ji Z L (2006). Cell wall degrading enzymes produced by Rhizoctonia solaniand their pathogenicity to rice plants. Jiangsu J Agr Sci, 22(1): 24–28 (in Chinese)

3 Deising H, Frittrang A K, Kunz S, Mendgen K ,(1995). Regulation of pectin methylesterase and polygalacturonate lyase activity during differentiation of infection structures in Uromyces viciae-fabae. Microbiology, 141:561–571

4 Fan Z H, Shen R X, Zhou Z M. (1989). The relationships between the activities of peroxidase and phynelalanine ammonialyase and the resistance of poplar to leaf rust. Acta Phytopathol Sin, 19(2): 95–100 (in Chinese)

5 Feng J, Gao Z G, Xue C S, Zhuang J H, Chen J, Bai S Y (2002). The pathogenesis of the cell-degrading enzymes produced by Curvularia lunata. Rain Fed Crop, 22(3): 164–166 (in Chinese)

6 Heiler S, Mendgen K, Deising H (1993). Cellulotic enzymes of the obligately biotrophic rust fungus Uromyces viciae-fabaeare regulated differentiation-specifically. Mycol Res, 97: 77–85

7 Larson P R, Isebrands J G. (1971). The plastochron index as applied to developmental studies of cottonwood. Can J For Res, 1: 1–11

8 Li B J, Chen L Q, Meng W J, Wang F J (2003). Effect of temperature and humidity on cell wall degrading enzymes produced by Botrytis cinereaduring infection of tomato plant. Acta Phytopathol Sin, 33(3): 209–212 (in Chinese)

9 Li B J, Zhou C L, Zhao K H, Li F Y (2000). Pathogenic mechanism of scab of cucumber caused by Cladosporium cucumerinum II: The cell wall degrading enzymes and its pathogenic action. Acta Phytopathol Sin, 30(1): 13–18 (in Chinese)

10 Lu X F (2005). Study on Proteins in Interaction System between Wheat and Stripe Rust Fungi. Yangling: Northwest A & F University (in Chinese)

11 Pei M H, McCracken A R (2005). Rust Diseases of Willow and Poplar. CABI Publishing, 1–264

12 Tian C M, Li Z Q, Kang Z S (1999). Advances in researches of cathay poplar leaf rust (Melampsora larici populina Kleb.). J Northwest For Coll, 14(2): 81–88 (in Chinese)

13 Tian C M, Liang Y M, Kang Z S, Li Z Q, Zhao Y X (2002). Ultrastructure of poplar leaf Infected by rust fungus (Melampsora larici-populina Kleb.). Acta Phytopathol Sin, 32(1): 71–78 (in Chinese)

14 Wei Y N (1984). A study on activities of polyphenol oxidase and peroxidase, and tendency in changes of isozyme spectra in Chinese white poplar (Populus tomentosa Carr.) leaves after infected with Melompsora magnusianaWagner. J Beijing For Univ, 17(3): 73–92 (in Chinese)

15 Xu H, Mendgen K (1997). Targeted cell wall degradation at the penetration site of cowpea rust basidiosporelings. Am Phytopathol Soc, 10(1): 87–94

16 Zhao L, Zhang T Y (2002). The role of degrading enzymes in plant-pathogen interaction. Microbiology, 29(1): 89–93 (in Chinese)