AFLP analysis of Fusarium circinatum and relative species

Abstract

AFLP fingerprinting analysis of Fusarium circinatum, Nirenberg and O’Donnell and relative species was carried out. Ten primer-pairs that could generate abundant polymorphism fragments were screened. A total of 298 nucleotide acid fragments were amplified with the primers from the template of the 17 strains of Fusarium spp., among which 283 fragments were polymorphic. Percentage of polymorphic loci produced by each pair of AFLP primer-pair was 94.97% in average and varied from 89.29% to 100%. All these data indicated that considerable genetic variation existed among F. circinatum and relative species at DNA level. Molecular genetic distances among Fusarium spp. were calculated, and the relationship among them was described quantitatively. Compared with biological species, the result of cluster analysis was basically similar to the phenotypic classification of species. Genetic diversity of E-AT/M-CAA AFLP fingerprinting of Fusarium spp. was analyzed, and specific and difference bands for each species and all Fusarium section Liseola tested were identified based on the E-AT/M-CAA AFLP fingerprinting.

Keywords

Fusarium circinatum / AFLP / genetic relationship / identification