Molecular detection and drought resistance analysis of B-transgenic poplars ( × )

Abstract

The propagation of drought-resistant transgenic poplars is one of the more effective ways to improve the ecological state of arid regions. The expression of SacB gene in transgenic lines of Populus alba × P. glandulosa was analyzed by a semi-quantitative RT-PCR. Drought resistance of these transgenic lines was evaluated by water stress trials in a greenhouse. Results showed that the mRNA transcription of SacB was found in all transgenic lines and fructan has accumulated in the leaves of these plants. The growth, biomass and leaf water content of some transgenic lines were significantly higher than those of the control plants under drought stress. Correlation analysis indicated that the said factors were significantly and positively related to the concentration of fructan in the leaves of the transgenic lines. Results revealed that the expression of SacB gene in transgenic poplars enhanced its resistance to water stress.

References

1. Altman A Alegrand T Pelah D Tzfira T Vinocur B Wang W Yarnitzky O 1996 Molecularbiology of drought tolerance and transformation of Populus and Pinus at the Hebrew University of JerusalemDendrom3(2)57
2. Arisi A C M Cornic G Jouanin L Foyer C H 1998 Overexpression of iron siperoxide dismutase in transformed poplarmodifies the regulation of photosynthesis at low CO₂ partial pressures of following exposure to the prooxidant herbicidemethyl viologenPlant Physiol117565574. doi:10.1104/pp.117.2.565
3. Cheng S H Xie C H Liu J 2002 Recent progress in studies on fructansin higher plantsChin Bull Bot19(2)204212 (in Chinese)
4. Ebskamp M J M Van der Meer I M Spronk B A Weisbeek P J Smeekens S C M 1994 Accumulationof fructose polymers in transgenic tobaccoBiotechnology12(3)272275. doi:10.1038/nbt0394‐272
5. Hendry G F 1993 Evolutionary origins and natural fractions of fructans:a climatological, biogeographic and mechanistic appraisa1New Phytol123314. doi:10.1111/j.1469‐8137.1993.tb03753.x
6. Jiang D Yu Z D Li Y G Han H Y Yu S L 2000 Study on fructans metabolismin stems of high-yielding winter wheat and effects of nitrogen levelsapplied on fructans metabolismActa AgronSin28(1)7985 (in Chinese)
7. Nicolescu C Sandre C Jouanin L Chriqui D 1996 Genetic engineering of phenolic metabolism in poplar in relationwith resistance against pathogensActa BotGallica43539546
8. Pilon-Smits E A H Ebskamp M J M Paul M J Jeuken M J W Weisbeek P J Smeekens S C M 1995 Improved performance of transgenic fructan-accumulatingtobacco under drought stressPlant Physiol107125130
9. Pilon-Smits E A H Terry N Sear S Van Dun K 1999 Enhanced drought resistance in fructan-producing sugar beetPlant Physiol Biochem37313317. doi:10.1016/S0981‐9428(99)80030‐8
10. Sprenger N Schellenbaum L Van Dun K 1997 Fructan synthesis in transgenic tobaccoand chicory plants expressing barley sucrose: fructan 6-fructosyltansferaseFebs Lett400355358. doi:10.1016/S0014‐5793(96)01418‐4
11. Steinmetz M Aymerich S 1986 Genetic analysisof sacR, a cis-regulator of levan-saccharase synthesis of Bacillus subtilisAnn Inst Pasteur Microbiol137(1)314. doi:10.1016/S0769‐2609(86)80001‐1
12. Steinmetz M Le C D Aymerich S Gonzy-Tréboul G Gay P 1985 The DNA sequenceof the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sitesMol Gen Genet200(2)220228. doi:10.1007/BF00425427
13. Van der Meer I M Ebskamp M Visser R Weisbeek P J Smeekens S 1994 Fructan as a new carbohydratesink in transgenic potato plantsPlant Cell6561570. doi:10.1105/tpc.6.4.561
14. Vijn I van Dijken A Sprenger N 1997 Fructan of the inulin neoseries is synthesizedin transgenic chicory plants (Cichorium intybus L.) Harbouring onion (Alliumcepa L.) fructan: fructan 6 G-fructosyltransferase. Plant J11387397. doi:10.1046/j.1365‐313X.1997.11030387.x
15. Wang G L Li T S Fang H J Hu Y L Zhao H W Lin Z P 2004 Obtainment of transgenic tomato of levansucrase gene withresistance to low temperatureSci AgricSin37(8)11931197
16. Wang W X Tzfira T Levin V Shoseyov O Altman A 1998 Plant tolerance to waterand salt stress: the expression pattern of a water stress responsiveprotein (BspA) in transgenic aspen plantsIn: Altman AZiv MIzhar SPlant Biotechnology and in vitro Biology in the 21st Century.DordrechtKluwer Academic Publishers
17. Wen S K Dong C H Yin C Y 1997 Introduction of phytohormone biosyntheticgenes to Populus tomentosa andestablishment of genetic transformation systemBiotechnology7(2)1114
18. Yang J S Wang G Q 1995 Silencingand negative of transgenicProg Biotech15(3)4145
19. Zhang B Y Su X H Huang Q J Zhang X H Hu Z M 2005 Regeneration of transgenicpoplar (Populus alba × P. glandulosa) expressing levansucrase from Bacillus subtilis.Sci Silv Sin41(3)4853
20. Zhang H Dong W Zhou J M Du B X Gu D M Chen S Y 1998 The cloning of levansucrase gene and its engineering ofsalt tolerant tobacco plantsChin J Biotech14(2)181186
21. Zhang Z B Shan L 1999 Advance indrought resistance transgenic cropWorldSci-Tech R&D21(2)3133