Study of two-dimensional liquid chromatography with high temperature NPLC and room temperature RPLC

Abstract

To overcome the peak band broadening and to increase the peak capacity and separation efficiency of a two-dimensional liquid chromatographic system, a high-temperature normal phase liquid chromatography (HTNPLC) was used as the first dimension (1^st-D), and a RPLC was used as the second dimension (2^nd-D). The sample was first separated on the 1^st-D CN column and the primary eluent stored in the sampling-loop system alternatively (in HTNPLC×RPLC mode) or selectively (in HTNPLC/RPLC mode) and was then transferred to 2^nd-D C₁₈ column for further separation. The resolution and separation efficiency of the systems were greatly improved. The systems were evaluated by analyzing several polycyclic aromatic hydrocarbons and Glycyrrhiza uralensis extract.

Keywords

two-dimensional liquid chromatography / HTNPLC /RPLC / sample loops- valve interface / traditional Chinese medicine / Glycyrrhiza uralensis

References

[1] Moore A W, Jorgenson J W. Comprehensive three-dimensional separation of peptides using size exclusion chromatography/ reversed phase liquid chromatography/optically gated capillary zone electrophoresis. Anal Chem, 1995, 67: 3456-3463

[2] Haefliger O P. Universal two-dimensional HPLC technique for the chemical analysis of complex surfactant mixtures. Anal Chem, 2003, 75: 371-378

[3] Murahash I T. Comprehensive two-dimensional high-performance liquid chromatography for the separation of polycyclic aromatic hydrocarbons. Analyst, 2003, 128 (6): 611-615

[4] Patrick H L, Victor I. Parametric modulation in liquid chromatography: multivariate optimization of mobile phase composition and temperature. Anal Chem, 1997, 69: 2963-2971

[5] Sheng G C, Shen Y F, Lee M L. Elevated temperature liquid chromatography using reversed-phase packed capillary columns. J Microcol Sep, 1997, 9: 63-72

[6] Kephart T S, Dasgupta P K. Superheated water eluent capillary liquid chromatography. Talanta, 2002, 56: 977-987

[7] Giddings J C. Two-dimensional separations: concept and promise. Anal Chem, 1984, 56: 1258A-1270A

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