Thermodynamic analysis of fluorescence enhancement and Quenching theory equations

Abstract

The action of the three kinds of new third generation cephalosporin-class drugs, cefepime hydrochroride, cefpiramide and ceftizoxime with HSA and BSA was studied at different temperatures through the fluorescence method. First, the binding constants were calculated by using fluorescence quenching and enhancement theoretical equations. Their thermodynamic functions were also calculated. Because the K_A corresponding to the different theoretical equations are not completely the same, the thermodynamic parameters calculated are also different. In this paper, the differences among these thermodynamic data obtained from the different theoretical equations were analyzed and the results show that the thermodynamic data deduced from fluorescence enhancement are more reasonable. Thus, we propose that even when the fluorescence quenching action of the acceptor-substrate is studied, more realistic data can be obtained by using the fluorescence enhancement equation.

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