Electrochemical behaviors of ofloxacin and its voltammetric determination at carbon nanotubes film modified electrode

Abstract

A multi-wall carbon nanotubes (MWNTs)-Nafion film-coated glassy carbon electrode (GCE) was fabricated and the electrochemical behavior of ofloxacin on the MWNTs-Nafion film-coated GCE were investigated by cyclic voltammetry (CV), linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS). The oxidation peak current of ofloxacin increased significantly on the MWNTs-Nafion film modified GCE compared with that using a bare GCE. This nano-structured film electrode exhibited excellent enhancement effects on the electrochemical oxidation of ofloxacin. A well-defined oxidation peak attributed to ofloxacin was observed at 0.97 V and was applied to the determination of ofloxacin. The oxidation peak current was proportional to ofloxacin concentration in the ranges 1.0 × 10^-8 to 1.0 × 10^-6 mol/L and 1.0 × 10^-6 to 2.0 × 10^-5 mol/L. A detection limit of 8.0 × 10^-9 mol/L was obtained for 400 s accumulation at open circuit (S/N = 3). This method for the detection of ofloxacin in human urine was satisfactory.

References

1. Zhang S M Du Y K Li Y K Determination of ofloxacin by alternating current oscillopolarographictitrationChinese J Anal Chem 1998 26(7)915
2. Espinosa-Mansilla A de la Pena A M Gomez D G Salinas F HPLC determinationof enoxacin, ciprofloxacin, norfloxacin and ofloxacin with photoinducedfluorimetric (PIF) detection and multiemission scanning-Applicationto urine and serumJournal of ChromatographyB-Analytical Technologies in the Biomedical and Life Sciences 2005 822(1–2)185193. doi:10.1016/j.jchromb.2005.05.045
3. Shervington L A Abba M Hussain B Donnelly J The simultaneousseparation and determination of five quinolone antibotics using isocraticreversed-phase HPLC: Application to stability studies on an ofloxacintablet formulationJournal of Pharmaceuticaland Biomedical Analysis 2005 39(3–4)755769
4. Tong C L Xiang G H Huang D J Liu W P Determinationof ofloxacin by the terbium(III) ion fluorescence probe sensitizedby the surfactantChinese J Anal Chem 2004 32(5)619621
5. Gong Q J Jin W J Dong C Recognition and determination of ofloxacin enantiomersby synchronization-derivative fluorimetryChinese J Anal Chem 2000 28(6)672677
6. Francis Paul S Adcock Jacqui L Chemiluminescence methods forthe determination of ofloxacinAnalyticaChimica Acta 2005 541(1–2)312. doi:10.1016/j.aca.2004.11.030
7. GAO P SHI J LI J N Liu S M Anodic adsorptivevoltammetric determination of ofloxacin at a carbon paste electrodeChin J Appl Chem 2005 22(5)578580
8. Radi A El-Sherif Z Determination of levofloxacinin human urine by adsorptive square-wave anodic stripping voltammetryon a glassy carbon electrodeTalanta 2002 58319324. doi:10.1016/S0039‐9140(02)00245‐X
9. Radi A El Ries M A Kandil S Electrochemical study of the interaction of levofloxacinwith DNAAnalytica Chimica Acta 2003 4956167. doi:10.1016/j.aca.2003.08.018
10. Britto P J Santhanam K S V Ajayan PM Carbon nanotube electrode for oxidation of dopamineB ioelectrochem B ioenerg 1996 41121125
11. Tsang S C Chen Y K Harris P J E Green M L H A Simplechemical method of opening and filling carbon nanotubesNature 1994 372159162. doi:10.1038/372159a0
12. Gerhardt G A Oke A F Nage Moghaddam G B Adams R N Nafion-coatedelectrodes with high selectivity for CNS electrochemistryBrain Res 1984 290390395. doi:10.1016/0006‐8993(84)90963‐6
13. Wu K b Fei J J Hu S S Simultaneous determination of dopamine and serotonin onthe glassy carbon electrode coated with carbon nanotubes filmAnal Biochem 2003 318100106. doi:10.1016/S0003‐2697(03)00174‐X
14. Wang G Xu J J Chen H Y Interfacing cytochromec to electrodes with a DNA-carbonnanotube composite filmElectrochemistryCommunication 2002 4(6)506509
15. Kauffmann J M Nunez-vergara L J Vire J C Squella L A Patriarche G J Voltammetric oxidation of trazodoneElectrochimica Acta 1987 32(8)11591162. doi:10.1016/0013‐4686(87)80028‐2