Study on spectroscopy of two flavonyl glycosides from

Abstract

To study the bioactive constituents from Andrographis paniculata, two compounds were isolated and purified by column chromatography on AB-8 macroporous adsorption resin and polyamide. Their structures were determined by various spectroscopic methods, including UV, FABMS, ¹H-NMR, ¹³C-NMR, DQFCOSY, TOCSY, HMQC, HMBC, and NOESY. The ¹H-NMR and ¹³C-NMR signals of the two compounds were assigned. The structures of the two compounds were elucidated as 5,4′-dihydroxy-7-methoxyflavone-6-yl-?-D-glucopyranoside and 5,4′-dihydroxy-7-methoxyflavone- 8-yl-?-D-glucopyranoside, respectively.

Keywords

Andrographis paniculata / 5,4& / #8242 / -dihydroxy-7-methoxyflavone-6-yl-β-D-glucopyranoside / 5,4& / #8242 / -dihydroxy-7- methoxyflavone-8-yl-β-D-glucopyranoside / NMR / structure analysis