Nuclease activity of copper(II) complex containing 1,10-phenanthroline-5,6-dione and L-phenylalanine ligands

Abstract

The interaction between CT-DNA and a ternary copper (II) complex, [Cu(phendio)(L-Phe)(H₂O)](ClO₄)·H₂O (CuPP, phendio = 1,10-phenanthroline-5,6-dione, L-Phe = L-phenylalanine), has been conducted by electronic spectra, fluorescence spectroscopy and cyclic voltammetry. It has been found that the max. absorption peak from the electronic spectra is red shifted and the intensity is weakened and that the values of peak current from cyclic voltammetry are decreased significantly in the presence of DNA compared with that in the absence of DNA. At the same time, the complex can quench the emission intensity of EB-DNA system. The existence of the intercalation mode between the complex and DNA was proven. By submarine gel electrophoresis, we found that the copper(II) complex can cleave circular plasmid pBR322 DNA into nicked and linear forms in the presence of ascorbic acid and H₂O₂.

References

1. Erkkilia K E Odom D T Barton K J Recognition and reaction of etallointercalators with DNAChem Rev 1999 9927772795. doi:10.1021/cr9804341
2. Sigman D S Mazumder A Perrin D M Chemical nucleasesChem Rev 1993 9322952316. doi:10.1021/cr00022a011
3. Huang S G Martin K Fluorescent nucleotide derivativesas specific probes for the uncoupling protein: thermodynamics andkinetics of binding and the control by pHBiochem 1995 305349360
4. Ren R Yang P Zheng W A simple copper(II)-L-histidine system for efficient hydrolyticcleavage of DNAInorg Chem 2000 3954545463. doi:10.1021/ic0000146
5. Reichmann M E Rice S A Thomas C A A further examination of the molecular weight and sizeof desoxypentose nucleic acidJ Am ChemSoc 1954 7630473055. doi:10.1021/ja01640a067
6. Xu G J Kou Y Y Feng L Yan S P Liao D Z Cheng P Cu(II)and Ni(II)-1,10-phenanthroline- 5,6-dione-amino acid ternary complexesexhibiting pH-sensitive redox propertiesAppl Organometal Chem 2006 20351356. doi:10.1002/aoc.1064
7. Wolfe A Shimer G H Meehan T Polycyclic aromatic hydrocarbons physically intercalateinto duplex regions of denatured DNABiochemistry 1987 2663926396. doi:10.1021/bi00394a013
8. Barton J K Danishefsky A T Goldberg J M Tris(phenanthroline)ruthenium(II): stereoselectivity inbinding to DNAJ Am Chem Soc 1984 10621722176. doi:10.1021/ja00319a043
9. Tysoe A Morgan R J Baker A D Spectroscopic investigation of differential binding modesof DELTA- and LAMBDA-Ru(bpy)₂(ppz)²⁺ with calf thymus DNAJPhys Chem 1993 9717071714. doi:10.1021/j100110a038
10. Liu C Zhou J Li Q DNA damage by copper(II) complexes: coordination-structuraldependence of reactivitiesJ Inorg Biochem 1999 75233239. doi:10.1016/S0162‐0134(99)00037‐9
11. Zou X H Ye B H Li H Aluminum speciation studies in biological fluids: Part7. a quantitative investigation of aluminum(III)–malate complexequilibria and their potential implications for aluminum metabolismand toxicity. J Biol Inorg Chem 2001 6143150. doi:10.1007/s007750000183
12. Sedov A Dunaj-Jurco M Kabesova M The possibility of electronic spectra utilization for molecularstructure determination in the case of Cu(II) complexes with 2,2′-bipyridyland o-phenanthrolineInorg Chim Acta 1987 64L257263. doi:10.1016/S0020‐1693(00)90346‐3
13. Wolf A Shimer G H Meehan T Polycyclic aromatic hydrocarbons physically intercalateinto duplex regions of denatured DNABiochemistry 1987 2663926397
14. Baguley B C Lebret M Quenching of DNA-ethidium fluorescenceby amsacrine and other antitumor agents: a possible electron-transfereffectBiochemistry 1984 23937943. doi:10.1021/bi00300a022
15. Jenkins T C, Fox K R. Methods in Molecular Biology, vol. 90, drug-DNA interaction protocols, humanapress incTotowa, New Jersey 1997 205207
16. Pogozelski W K Tullius T D Oxidative strand scission ofnucleic acids: routes initiated by hydrogen abstraction from the sugarmoietyChem Rev 1998 9810891107. doi:10.1021/cr960437i
17. Humphreys K J Karlin K D Rokita S E Targeted strand scission of DNA substrates by a tricopper(II)coordination complexJ Am Chem Soc 2002 12480558066. doi:10.1021/ja012539i
18. Klinman J Mechanismswhereby mononuclear copper proteins functionalize organic substratesChem Rev 1996 9625412561. doi:10.1021/cr950047g