Kinetic analysis of interaction between lipopolysaccharide and biomolecules

Abstract

Lipopolysaccharide (LPS) is a major component of the outer membrane of all gram-negative bacteria. It interacts with some biomolecules and triggers a toxic reaction. In this paper, we studied the interaction between LPS from Salmonella Minnesota and some biomolecules using a surface plasmon resonance (SPR) biosensor. Biomolecules were immobilized on a CM5 sensor chip using the amino coupling method and LPS was injected over the immobilized surfaces. The affinity constant K_A of LPS with serum albumin, hemoglobin, chitosan and lysozyme was 2.36 × 10⁷, 2.03 × 10⁸, 7.58 × 10⁶, 2.82 × 10⁴ L·mol^-1, respectively. However, LPS could not interact with ferritin.

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