Environmental proteomics: a potential tool in wastewater-based epidemiology

Abstract

Wastewater-based epidemiology (WBE) is a new complementary alternative to traditional epidemiological surveillance approaches for community health surveys. It provides objective, flexible, and comprehensive data on diseases, exposure to toxicants and pathogens, social habits, and lifestyle. Wastewater is regarded as an integrated pooled sample of the entire population served by a certain wastewater system, thus providing an average picture of its health status. WBE relies on the extraction, analysis, and subsequent interpretation of appropriate health status indicators, including both chemical and biological components, either exogenous or endogenous, altogether constituting the exposome. Instrumental development, especially mass spectrometry (MS) for the chemical analysis of small and large molecules, has been critical to the success achieved through WBE. Whereas all previous studies have been restricted to small molecules, the use of proteomics in WBE is an obvious extension of the intensive ongoing pursuit of biomarkers for community surveillance of diseases and health status. The relevance of human proteins present in wastewater as potential WBE indicators of population-prevalent diseases has been previously postulated, although no real examples to demonstrate their feasibility have been available until recently. These findings provide new insights into the characterization of wastewater proteomics useful for practical purposes related not only to WBE applications but also to environmental monitoring and wastewater treatment plant management.

Keywords

Environmental proteomics / wastewater-based epidemiology / sewage epidemiology / biomarkers / water fingerprinting / mass spectrometry

References

[1] Winkelstein W.From the editor.Am J Epidemiol2001;154:93

[2] Vitale D,Picó Y.Wastewater-based epidemiology, a tool to bridge biomarkers of exposure, contaminants, and human health.Curr Opin Environ Sci Health2021;20:100229

[3] Daughton CG.Illicit drugs: contaminants in the environment and utility in forensic epidemiology. In: Whitacre DM, editor. Reviews of Environmental Contamination and Toxicology Volume 210. New York: Springer; 2011. pp. 59-110.

[4] Sims N.Future perspectives of wastewater-based epidemiology: monitoring infectious disease spread and resistance to the community level.Environ Int2020;139:105689 PMCID:PMC7128895

[5] Daughton CG.Monitoring wastewater for assessing community health: Sewage Chemical-Information Mining (SCIM).Sci Total Environ2018;619-620:748-64 PMCID:PMC6091531

[6] Rice J.A new paradigm in public health assessment: Water fingerprinting for protein markers of public health using mass spectrometry.TRAC-Trend Anal Chem2019;119:115621

[7] Vermeulen R,Barabási AL.The exposome and health: where chemistry meets biology.Science2020;367:392-6 PMCID:PMC7227413

[8] Wild CP.Complementing the genome with an “exposome”: the outstanding challenge of environmental exposure measurement in molecular epidemiology.Cancer Epidemiol Biomarkers Prev2005;14:1847-50

[9] Picó Y.Mass spectrometry in wastewater-based epidemiology for the determination of small and large molecules as biomarkers of exposure: toward a global view of environment and human health under the COVID-19 outbreak.ACS Omega2021;6:30865-72 PMCID:PMC8613814

[10] Andrés-costa MJ,Picó Y.Liquid chromatography-mass spectrometry as a tool for wastewater-based epidemiology: assessing new psychoactive substances and other human biomarkers.TrAC Trends Anal Chem2017;94:21-38

[11] Boogaerts T,Covaci A,van Nuijs ALN.Analytical method for the simultaneous determination of a broad range of opioids in influent wastewater: optimization, validation and applicability to monitor consumption patterns.Talanta2021;232:122443

[12] Kinyua J,Rousis NI.Investigation of biotransformation products of p-methoxymethylamphetamine and dihydromephedrone in wastewater by high-resolution mass spectrometry.Metabolites2021;11:66 PMCID:PMC7912097

[13] Andrés-Costa MJ,Picó Y.Analysis of psychoactive substances in water by information dependent acquisition on a hybrid quadrupole time-of-flight mass spectrometer.J Chromatogr A2016;1461:98-106

[14] Mastroianni N,Postigo C,López de Alda M.Five-year monitoring of 19 illicit and legal substances of abuse at the inlet of a wastewater treatment plant in Barcelona (NE Spain) and estimation of drug consumption patterns and trends.Sci Total Environ2017;609:916-26

[15] Thomas KV,Castiglioni S.Comparing illicit drug use in 19 European cities through sewage analysis.Sci Total Environ2012;432:432-9

[16] Burgard DA,Field JA.Working upstream: how far can you go with sewage-based drug epidemiology?.Environ Sci Technol2014;48:1362-8

[17] Gao J,Du P.Measuring selected PPCPs in wastewater to estimate the population in different cities in China.Sci Total Environ2016;568:164-70

[18] Castiglioni S,Borsotti A,Zuccato E.A novel approach for monitoring tobacco use in local communities by wastewater analysis.Tob Control2015;24:38-42

[19] Ryu Y,Barron LP.Comparative measurement and quantitative risk assessment of alcohol consumption through wastewater-based epidemiology: An international study in 20 cities.Sci Total Environ2016;565:977-83

[20] Rousis NI,Zuccato E.Wastewater-based epidemiology to assess pan-European pesticide exposure.Water Res2017;121:270-9

[21] Lopardo L,Proctor K,Barden R.Estimation of community-wide exposure to bisphenol A via water fingerprinting.Environ Int2019;125:1-8

[22] Ryu Y,Bade R.Increased levels of the oxidative stress biomarker 8-iso-prostaglandin F(2α) in wastewater associated with tobacco use.Sci Rep2016;6:39055 PMCID:PMC5157025

[23] Alygizakis N,Rousis NI.Analytical methodologies for the detection of SARS-CoV-2 in wastewater: protocols and future perspectives.Trends Analyt Chem2021;134:116125 PMCID:PMC7677696

[24] Barceló D.Wastewater-Based Epidemiology to monitor COVID-19 outbreak: present and future diagnostic methods to be in your radar.Case Stud Therm Eng2020;2:100042

[25] Carrascal M,Ginebreda A.Discovery of large molecules as new biomarkers in wastewater using environmental proteomics and suitable polymer probes.Sci Total Environ2020;747:141145

[26] Perez-lopez C,Carrascal M,Abian J.Non-target protein analysis of samples from wastewater treatment plants using the regions of interest-multivariate curve resolution (ROIMCR) chemometrics method.J Environ Chem Eng2021;9:105752

[27] Casanovas A,Abián J,Llobera M.Discovery of lipoprotein lipase pI isoforms and contributions to their characterization.J Proteomics2009;72:1031-9

[28] Kuhn R,Rapp E,Palese LL.Metaproteome analysis of sewage sludge from membrane bioreactors.Proteomics2011;11:2738-44

[29] Westgate PJ.Evaluation of proteins and organic nitrogen in wastewater treatment effluents.Environ Sci Technol2010;44:5352-7

[30] Zhang P,Xu XY,Dai YZ.Identification and function of extracellular protein in wastewater treatment using proteomic approaches: a minireview.J Environ Manage2019;233:24-9

[31] Mattes W,Orr MS,Mendrick DL.Biomarkers of tobacco smoke exposure. Elsevier; 2014. pp. 1-45.