Titin gene mutations enhance radiotherapy efficacy via modulation of tumour immune microenvironment in rectum adenocarcinoma

Abstract

	1. The association between TTN mutations and tumour mutation burden, as well as immune cell infiltration in READ, is examined.
	2. TTN mutations enhance the radiation sensitivity of READ cells and weaken DNA damage repair in response to radiation.
	3. TTN mutations increase the radiation sensitivity of READ cells by inhibiting ANKRD1.
	4. The infiltration of CD8+ and CD4+ T cells induced by TTN mutations is essential for anti-tumour immunity.
	5. TTN mutations serve as a biomarker for the pathological response to preoperative radiotherapy in READ.

Keywords

ANKRD1 / anti-tumour effects / DNA damage repair / radiotherapy sensitivity / rectum adenocarcinoma / T-cell infiltration / TTN mutations

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2025 The Author(s). Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.