Screening for metastasis-related genes in mouse melanoma cells through sequential tail vein injection
Biophysics Reports ›› 2024, Vol. 10 ›› Issue (1) : 15 -21.
Screening for metastasis-related genes in mouse melanoma cells through sequential tail vein injection
), Yan Wang4,*(), Chengqin Wang6,*(), Yujie Lei7,*(), Yan Chen3,*(), Junling Shen1,*(), Lei Sang1,*(), Yan Bai1,*(), Jianwei Sun1,*()
Tumor metastasis, responsible for approximately 90% of cancer-associated mortality, remains poorly understood. Here in this study, we employed a melanoma lung metastasis model to screen for metastasis-related genes. By sequential tail vein injection of mouse melanoma B16F10 cells and the subsequently derived cells from lung metastasis into BALB/c mice, we successfully obtained highly metastatic B16F15 cells after five rounds of in vivo screening. RNA-sequencing analysis of B16F15 and B16F10 cells revealed a number of differentially expressed genes, some of these genes have previously been associated with tumor metastasis while others are novel discoveries. The identification of these metastasis-related genes not only improves our understanding of the metastasis mechanisms, but also provides potential diagnostic biomarkers and therapeutic targets for metastatic melanoma.
Melanoma / Lung metastasis model / Gene screening / Tail vein injection
/
| 〈 |
|
〉 |
PDF
(933KB)
