Microbial mechanisms of organic matter mineralization induced by straw in biochar-amended paddy soil

Abstract

Combined straw and straw-derived biochar input is commonly applied by farmland management in low-fertility soils. Although straw return increases soil organic matter (SOM) contents, it also primes SOM mineralization. The mechanisms by which active microorganisms mineralize SOM and the underlying factors remain unclear for such soils. To address these issues, paddy soil was amended with ¹³C-labeled straw, with and without biochar (BC) or ferrihydrite (Fh), and incubated for 70 days under flooded conditions. Compound-specific ¹³C analysis of phospholipid fatty acids (¹³C-PLFAs) allowed us to identify active microbial communities utilizing the ¹³C-labeled straw and specific groups involved in SOM mineralization. Cumulative SOM mineralization increased by 61% and 27% in soils amended with Straw + BC and Straw + Fh + BC, respectively, compared to that with straw only. The total PLFA content was independent of the straw and biochar input. However, ¹³C-PLFAs contents increased by 35–82% after biochar addition, reflecting accelerated microbial turnover. Compared to that in soils without biochar addition, those with biochar had an altered microbial community composition-increased amounts of ¹³C-labeled gram-positive bacteria (¹³C-Gram +) and fungi, which were the main active microorganisms mineralizing SOM. Microbial reproduction and growth were susceptible to nutrient availability. ¹³C-Gram + and ¹³C-fungi increased with Olsen P but decreased with dissolved organic carbon and

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contents. In conclusion, biochar acts as an electron shuttle, stimulates iron reduction, and releases organic carbon from soil minerals, which in turn increases SOM mineralization. Gram + and fungi were involved in straw decomposition in response to biochar application and responsible for SOM mineralization.

Highlights

•	Straw return accelerates microbial mineralization of SOM.
•	Combined biochar and straw input raises SOM mineralization by 60%.
•	Biochar addition increases 13C incorporation from straw into Gram + bacteria and fungi.
•	13C-Gram + bacteria and 13C-fungi are the dominant active microorganisms that mineralize SOM.

Keywords

Soil organic matter / Rice straw return / Biochar application / Soil microbial community / ¹³C phospholipid fatty acid

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Funding

National Natural Science Foundation of China(42267050); Ningbo Municipal Bureau of Science and Technology(2022Z168); the “Pioneer” and “Leading Goose” R&D Program of Zhejiang(2022C02008)