Optimization of Clostridium beijerinckii semi-solid fermentation of rape straw to produce butyric acid by genome analysis

Abstract

Butyric acid is a volatile saturated monocarboxylic acid, which is widely used in the chemical, food, pharmaceutical, energy, and animal feed industries. This study focuses on producing butyric acid from pre-treated rape straw using simultaneous enzymatic hydrolysis semi-solid fermentation (SEHSF). Clostridium beijerinckii BRM001 screened from pit mud of Chinese nongxiangxing baijiu was used. The genome of C. beijerinckii BRM001 was sequenced and annotated. Using rape straw as the sole carbon source, fermentation optimization was carried out based on the genomic analysis of BRM001. The optimized butyric acid yield was as high as 13.86 ± 0.77 g/L, which was 2.1 times higher than that of the initial screening. Furthermore, under optimal conditions, non-sterile SEHSF was carried out, and the yield of butyric acid was 13.42 ± 0.83 g/L in a 2.5-L fermentor. This study provides a new approach for butyric acid production which eliminates the need for detoxification of straw hydrolysate and makes full use of the value of fermentation waste residue without secondary pollution, making the whole process greener and more economical, which has a certain industrial potential.

Keywords

Butyric acid / Clostridium beijerinckii / Semi-solid non-sterile fermentation / Rape straw / Genome annotation

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Funding

National Natural Science Foundation of China(31801522); the Cooperation Project of Wuliangye Group Co., Ltd. and Sichuan University of Science & Engineering, China(CXY2019ZR011); Sichuan University of Science & Engineering(2020RC36)