Fabrication of silver nanoparticles from leaf extract of Butea monosperma (Flame of Forest) and their inhibitory effect on bloom-forming cyanobacteria

Abstract

Background

Silver nanoparticles (SNPs) are used extensively in areas such as medicine, catalysis, electronics, environmental science, and biotechnology. Therefore, facile synthesis of SNPs from an eco-friendly, inexpensive source is a prerequisite. In the present study, fabrication of SNPs from the leaf extract of Butea monosperma (Flame of Forest) has been performed. SNPs were synthesized from 1% leaf extract solution and characterized by ultraviolet-visible (UV-vis) spectroscopy and transmission electron microscopy (TEM). The mechanism of SNP formation was studied by Fourier transform infrared (FTIR), and anti-algal properties of SNPs on selected toxic cyanobacteria were evaluated.

Results

TEM analysis indicated that size distribution of SNPs was under 5 to 30 nm. FTIR analysis indicated the role of amide I and II linkages present in protein in the reduction of silver ions. SNPs showed potent anti-algal properties on two cyanobacteria, namely, Anabaena spp. and Cylindrospermum spp. At a concentration of 800 μg/ml of SNPs, maximum anti-algal activity was observed in both cyanobacteria.

Conclusions

This study clearly demonstrates that small-sized, stable SNPs can be synthesized from the leaf extract of B. monosperma. SNPs can be effectively employed for removal of toxic cyanobacteria.

Keywords

Silver nanoparticles (SNPs) / Butea monosperma / Cyanobacteria / Anti-algal

References

[1.] Lengke FM, Fleet EM, Southam G. Biosynthesis of silver nanoparticles by filamentous cyanobacteria a from a silver(I) nitrate complex. Langmuir, 2007, 23: 2694-2699.

[2.] Prabhu S, Poulose EK. Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. Int Nano Lett, 2012, 2: 32.

[3.] Yamanaka M, Hara K, Kudo J. Synergistic bactericidal activity of silver nanoparticles and ciprofloxacin against phytopathogens. Appl Environ Microbiol, 2005, 71(11): 7589-7593.

[4.] Sadowski Z (2010) Biosynthesis and Application of Silver and Gold Nanoparticles, In: David Pozo Perez (Ed.), Silver Nanoparticles, Rijeka, Croatia, InTech, pp 257-276

[5.] Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotech Adv, 2009, 2: 776-83.

[6.] An C, Peng S, Sun Y. Facile synthesis of sunlight-driven AgCl:Ag plasmonic nanophotocatalyst. Adv Mater, 2010, 22(23): 2570-2574.

[7.] Wang RC, Gao YS, Chen SJ. Simple synthesis and size-dependent surface enhanced Raman scattering of Ag nanostructures on TiO2 by thermal decomposition of silver nitrate at low temperature. Nanotechnology, 2009, 20(37): 375605.

[8.] Horikoshi S, Abe H, Torigoe K, Abe M, Serpone N. Access to small size distributions of nanoparticles by microwave-assisted synthesis. Formation of Ag nanoparticles in aqueous carboxymethylcellulose solutions in batch and continuous-flow reactors. Nanoscale, 2010, 2(8): 1441-1447.

[9.] Peng Z, Spliethoff B, Tesche B, Walther T, Kleinermanns K. Laser-assisted synthesis of Au-Ag alloy nanoparticles in solution. J Phys Chem B, 2006, 2110(6): 2549-2554.

[10.] Murugan K, Senthilkumar B, Senbagam D, Al-Sohaibani S. Biosynthesis of silver nanoparticles using Acacia leucophloea extract and their antibacterial activity. Int J Nanomedicine, 2014, 9: 2431-2438.

[11.] Rahimi-Nasrabadi M, Pourmortazavi SM, Shandiz SA, Ahmadi F, Batooli H. Green synthesis of silver nanoparticles using Eucalyptus leucoxylon leaves extract and evaluating the antioxidant activities of extract. Nat Prod Res, 2014, 27: 1-6.

[12.] Rajasekharreddy P, Rani PU. Biofabrication of Ag nanoparticles using Sterculia foetida L. seed extract and their toxic potential against mosquito vectors and HeLa cancer cells. Mater Sci Eng C Mater Biol Appl, 2014, 39: 203-212.

[13.] Suresh G, Gunasekar PH, Kokila D, Prabhu D, Dinesh D, Ravichandran N, Ramesh B, Koodalingam A, Vijaiyan Siva G. Green synthesis of silver nanoparticles using Delphinium denudatum root extract exhibits antibacterial and mosquito larvicidal activities. Spectrochim Acta A Mol Biomol Spectrosc, 2014, 127: 61-66.

[14.] Burlia DA, Khadeb AB. A comprehensive review on Butea monosperma (Lam.) Kuntze. Pharmacogn Rev, 2007, 1: 333-7.

[15.] Pulz O, Gross W. Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol, 2004, 65: 155-165.

[16.] Rastogi RP, Richa SRP, Singh SP, Hader DP. Photoprotective compounds from marine organisms. J Ind Microbiol Biotechnol, 2010, 37: 537-558.

[17.] Singh SP, Kumari S, Rastogi RP, Singh KL, Richa SRP. Photoprotective and biotechnological potentials of cyanobacterial sheath pigment, scytonemin. Afr J Biotechnol, 2010, 9(5): 580-588.

[18.] Humpage AR, Hardy SJ, Moore EJ, Frosscio SM, Falcones IR. Microcystins (cyanobacterial toxin) in drinking water enhances the growth of aberrant cryptic foci in the mouse colon. J Toxicol Environ Health part A, 2000, 61: 155-165.

[19.] Park MH, Kim KH, Lee HH, Kim JS, Hwang SJ. Selective inhibitory potential of silver nanoparticles on the harmful cyanobacterium Microcystis aeruginosa. Biotechnol Lett, 2010, 32(3): 423-428.

[20.] Chaturvedi V, Chandravanshi M, Rahangdale M, Verma P (2013) An integrated approach of using polystyrene foam as an attachment system for growth of mixed culture of cyanobacteria with concomitant treatment of copper mine waste water. J Waste Management doi:10.1155/2013/282798

[21.] Henriques M, Silva A, Rocha J (2007) Extraction and Quantification of Pigments from a Marine Microalga: A Simple and Reproducible Method. In: Mendez-Vilas A. (Ed.), Communicating Current Research and Educational Topics and Trends in Applied Microbiology, Badajoz, Spain, Formatex, pp. 586–593.

[22.] Jodlowska S, Latala A. The comparison of spectrophotometric method and high-performance liquid chromatography in photosynthetic pigments analysis. OnLine J Bio Sci, 2011, 11(2): 63-69.

[23.] Markwell MA, Haas SM, Tolbert NE, Bieber LL. Protein determination in membrane and lipoprotein samples: manual and automated procedures. Methods Enz, 1981, 72: 296-303.

[24.] Chandran SP, Chaudhary M, Pasricha R, Ahmad A, Sastry M. Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract. Biotechnol Prog, 2006, 22: 577-583.

[25.] Huang Q, Li D, Sun Y, Lu Y, Su X, Yang H, Wang Y, Wang W, Shao N, Hong J, Chen C. Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf. Nanotechnology, 2007, 18: 105104.

[26.] Lalitha A, Subbaiya R, Ponmurugan P. Green synthesis of silver nanoparticles from leaf extract Azhadirachta indica and to study its anti-bacterial and antioxidant property. Int J Curr Microbiol App Sci, 2013, 2: 228-235.

[27.] Singhal G, Bhavesh R, Kasariya K, Sharma AR, Singh RP. Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. J Nanopar Res, 2011, 13: 2981-2988.

[28.] Mohanta YK, Behera SK (2014) Biosynthesis, characterization and antimicrobial activity of silver nanoparticles by Streptomyces sp. SS2. Bioprocess Biosyst Eng. doi:10.1007/s00449-014-1205-6

[29.] Ajitha B, Ashok Kumar Reddy Y, Sreedhara Reddy P. Biosynthesis of silver nanoparticles using Plectranthus amboinicus leaf extract and its antimicrobial activity. Spectrochim Acta A Mol Biomol Spectrosc, 2014, 128: 257-262.

[30.] Ashkarran AA, Bayat A. Surface plasmon resonance of metal nanostructures as a complementary technique for microscopic size measurement. Int Nano Lett, 2013, 3: 50.

[31.] Patil CD, Borase HP, Patil SV, Salunkhe RB, Salunke BK. Larvicidal activity of silver nanoparticles synthesized using Pergularia daemia plant latex against Aedes aegypti and Anopheles stephensi and nontarget fish Poecillia reticulate. Parasitol Res, 2012, 111(2): 555-562.

[32.] Gnanadesigan M, Anand M, Ravikumar S, Maruthupandy M, Syed Ali M, Vijayakumar V, Kumaraguru AK. Antibacterial potential of biosynthesized silver nanoparticles using Avicennia marina mangrove plant. Appl Nanosci, 2011, 2: 143-147.

[33.] Carmichael WW. Health effects of toxin producing cyanobacteria: the cyanoHABs. Hum Ecol Risk Assess, 2001, 7: 1393-1407.

[34.] Codd GA, Morrison LF, Metcalf JS. Cyanobacterial toxins: risk management for health protection. Tox Appl Phar, 2005, 203: 264-272.

[35.] Westrick J, Szlag D, Southwell B, Sinclair J. A review of cyanobacteria and cyanotoxins removal/inactivation in drinking water treatment. Anal Bional Chem, 2010, 397: 1705-1714.

[36.] Banerjee P, Satapathy M, Mukhopahayay A, Das P. Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Biores Bioproc, 2014, 1: 3.