Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis

Abstract

Background

In recent years, green synthesis of silver nanoparticles (AgNPs) has gained much interest from chemists and researchers. In this concern, Indian flora has yet to divulge innumerable sources of cost-effective non-hazardous reducing and stabilizing compounds utilized in preparing AgNPs. This study investigates an efficient and sustainable route of AgNP preparation from 1 mM aqueous AgNO₃ using leaf extracts of three plants, Musa balbisiana (banana), Azadirachta indica (neem) and Ocimum tenuiflorum (black tulsi), well adorned for their wide availability and medicinal property.

Methods

AgNPs were prepared by the reaction of 1 mM silver nitrate and 5% leaf extract of each type of plant separately. the AgNPs were duely characterized and tested for their antibacterial activity and toxicity.

Results

The AgNPs were characterized by UV-visible (vis) spectrophotometer, particle size analyzer (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and energy-dispersive spectroscopy (EDS). Fourier transform infrared spectrometer (FTIR) analysis was carried out to determine the nature of the capping agents in each of these leaf extracts. AgNPs obtained showed significantly higher antimicrobial activities against Escherichia coli (E. coli) and Bacillus sp. in comparison to both AgNO₃ and raw plant extracts. Additionally, a toxicity evaluation of these AgNP containing solutions was carried out on seeds of Moong Bean (Vigna radiata) and Chickpea (Cicer arietinum). Results showed that seeds treated with AgNP solutions exhibited better rates of germination and oxidative stress enzyme activity nearing control levels, though detailed mechanism of uptake and translocation are yet to be analyzed.

Conclusion

In totality, the AgNPs prepared are safe to be discharged in the environment and possibly utilized in processes of pollution remediation. AgNPs may also be efficiently utilized in agricultural research to obtain better health of crop plants as shown by our study.

Keywords

Green synthesis / Silver nanoparticles / SEM / TEM / Antimicrobial property / Toxicity

References

[1.] van den Wildenberg W. Roadmap report on nanoparticles, 2005, Barcelona, Spain: W&W Espana sl.

[2.] Gratzel M. Photoelectrochemical cells. Nature, 2001, 414: 338-344.

[3.] Okuda M, Kobayashi Y, Suzuki K, Sonoda K, Kondoh T, Wagawa A, Kondo A, Yoshimura H. Self-organized inorganic nanoparticle arrays on protein lattices. Nano Lett, 2005, 5: 991-993.

[4.] Dai J, Bruening ML. Catalytic nanoparticles formed by reduction of metal ions in multilayered polyelectrolyte films. Nano Lett, 2002, 2: 497-501.

[5.] Murray CB, Sun S, Doyle H, Betley T. Monodisperse 3d transition-metal (Co, Ni, Fe) nanoparticles. MRS Bull, 2001, 26: 985-991.

[6.] Bhattacharya R, Murkherjee P. Biological properties of “naked” metal nanoparticles. Adv Drug Deliv Rev, 2008, 60: 1289-1306.

[7.] Bhumkar DR, Joshi HM, Sastry M, Pokharkar VB. Chitosan reduced gold nanoparticles as novel carriers for transmucosal delivery of insulin. Pharm Res, 2007, 24: 1415-1426.

[8.] Sun Y, Yin Y, Mayers BT, Herricks T, Xia Y (2002) Uniform form silver nanowires synthesis by reducing AgNO₃ with ethylene glycol in presence of seeds and and poly(vinyl pyrrolidone). Chem Mater 14:4736-4745.

[9.] Yin B, Ma H, Wang S, Chen S. Electrochemical synthesis of silver nanoparticles under protection of poly (N-vinylpyrrolidone). J Phys Chem B, 2003, 107: 8898-8904.

[10.] Dimitrijevic NM, Bartels DM, Jonah CD, Takahashi K, Rajh T. Radiolytically induced formation and optical absorption spectra of colloidal silver nanoparticles in supercritical ethane. J Phys Chem B, 2001, 105: 954-959.

[11.] Callegari A, Tonti D, Chergui M. Photochemically grown silver nanoparticles with wavelength-controlled size and shape. Nano Lett, 2003, 3: 1565-1568.

[12.] Zhang L, Shen YH, Xie AJ, Li SK, Jin BK, Zhang QF. One-step synthesis of monodisperse silver nanoparticles beneath vitamin E Langmuir monolayers. J Phys Chem B, 2006, 110: 6615-6620.

[13.] Swami A, Selvakannan PR, Pasricha R, Sastry M. One-step synthesis of ordered two dimensional assemblies of silver nanoparticles by the spontaneous reduction of silver ions by pentadecylphenol Langmuir monolayers. J Phys Chem B, 2004, 108: 19269.

[14.] Naik RR, Stringer SJ, Agarwal G, Jones S, Stone MO. Biomimetic synthesis and patterning of silver nanoparticles. Nat Mater, 2002, 1: 169-172.

[15.] Gardea-Torresdey JL, Gomez E, Peralta-Videa JR, Parsons JG, Troiani H, Jose-Yacaman M, . Alfalfa sprouts: a natural source for the synthesis of silver nanoparticles. Langmuir, 2003, 19: 1357-1361.

[16.] Bar H, Bhui DK, Sahoo GP, Sarkar P, De SP, Misra A. Green synthesis of silver nanoparticles using latex of Jatropha curcas. Colloids Surf A Physicochem Eng Asp, 2009, 339: 134-139.

[17.] Chandran SP, Chaudhary M, Pasricha R, Ahmad A, Sastry M. Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract. Biotechnol Prog, 2006, 22: 577-583.

[18.] Krishnaraj C, Jagan EG, Rajasekar S, Selvakumar P, Kalaichelvan PT, Mohan N. Synthesis of silver nanoparticles using Acalypha indica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surf B: Biointerfaces, 2010, 76: 50-56.

[19.] Veerasamy R, Xin TZ, Gunasagaran S, Xiang TFW, Yang EFC, Jeyakumar N, Dhanaraj SA. Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. J Saudi Chem Soc, 2010, 15: 113-120.

[20.] Ghaffari-Moghaddam M, Hadi-Dabanlou R. Plant mediated green synthesis and antibacterial activity of silver nanoparticles using Crataegus douglasii fruit extract. J Indus Eng Chem, 2014, 20: 739-744.

[21.] Ghaffari-Moghaddam M, Hadi-Dabanlou R, Khajeh M, Rakhshanipour M, Shameli K. Green synthesis of silver nanoparticles using plant extracts. Korean J Chem Eng, 2014, 31: 548-557.

[22.] Bao Q, Zhang D, Qi P. Synthesis and characterization of silver nanoparticle and graphene oxide nanosheet composites as a bactericidal agent for water disinfection. J Colloid Interface Sci, 2011, 360: 463-470.

[23.] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem, 1951, 193: 265-275.

[24.] Chandlee JM, Scandalios JG. Analysis of variance affecting the catalase development programme in maize scutellum. Theor Appl Genet, 1984, 69: 71-77.

[25.] Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem, 1971, 44: 276-287.

[26.] Cipollini DF. The induction of soluble peroxidase activity in bean leaves by wind-induced mechanical perturbation. Amer J Bot, 1998, 85: 1586-1591.

[27.] Shukla VK, Pandey S, Pandey AC. Green synthesis of silver nanoparticles using neem leaf (Azadirachta indica) extract. Proceedings of International Conference On Advanced Nanomaterials And Nanotechnology, 2010, Guwahati, Assam (India): ICANN‐2009.

[28.] Namratha N, Monica PV. Synthesis of silver nanoparticles using Azadirachta indica (Neem) extract and usage in water purification. Asian J Pharm Tech, 2013, 3: 170-174.

[29.] Lalitha A, Subbaiya R, Ponmurugan P. Green synthesis of silver nanoparticles from leaf extract Azhadirachta indica and to study its anti-bacterial and antioxidant property. Int J Curr Microbiol App Sci, 2013, 2: 228-235.

[30.] Singhal G, Bhavesh R, Kasariya K, Sharma AR, Singh RP. Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. J Nanoparticle Res, 2011, 13: 2981-2988.

[31.] Philip D, Unni C. Extra cellular biosynthesis of gold and silver nanoparticles using Krishna tulsi (Ocimum sanctum) leaf. Phys E, 2011, 43: 1318-1322.

[32.] Siddiqui BS, Afshan F, Faizi GS, Naqui SNH, Tariq RM. Two insecticidal tetranortriterpenoids from Azadirachta indica. Phytochemistry, 2000, 53: 371-376.

[33.] Huang Q, Li D, Sun Y, Lu Y, Su X, Yang H, Wang Y, Wang W, Shao N, Hong J, Chen C. Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf. Nanotechnol, 2007, 18: 105104.

[34.] Shankar SS, Rai A, Ahmad A, Sastry M. Rapid synthesis of Au, Ag, and bimetallic Au core Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. J Colloid Interface Sci, 2004, 275: 496-502.

[35.] Song JY, Kim BS. Rapid biological synthesis of silver nanoparticles using plant leaf extract. Bioprocess Biosyst Eng, 2009, 32: 79-84.

[36.] Rout Y, Behera S, Ojha AK, Nayak PL. Green synthesis of silver nanoparticles using Ocimum sanctum (Tulashi) and study of their antibacterial and antifungal activities. J Microbiol Antimicro, 2012, 4: 103-109.

[37.] Zheng L, Hong FS, Lu SP, Liu C. Effect of nano-TiO₂ on strength of naturally and growth aged seeds of spinach. Biol Trace Elem Res, 2005, 104: 83-91.

[38.] Bashir F, Mahmooduzzafar STO, Iqbal M. The antioxidative response system in Glycine max (L.) Merr: exposed to Deltamethrin, a synthetic pyrethroid insecticide. Environ Poll, 2007, 147: 94-100.

[39.] Malik CP, Singh MB. Plant enzymology and histoenzymology, 1980, New Delhi, India: Kalyani Publishers.

[40.] Daniel SCGK, Kumar R, Sathish V, Sivakumar M, Sunitha S, Sironmani TA. Green synthesis (Ocimum tenuiflorum) of silver nanoparticles and toxicity studies in Zebra Fish (Danio rerio) model. Int J NanoSci Nanotechnol, 2011, 2: 103-117.