Isolation of four carbapenem-resistant gram-negative species from a single fly

doi:10.1186/s44149-024-00110-5

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Animal Disease ›› 2024, Vol. 4 ›› Issue (1) : 4. DOI: 10.1186/s44149-024-00110-5

Hanyu Wang¹, Hongwei Zhou¹, Gongxiang Chen¹, Ning Dong¹^,²^,³^,⁴()()

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Abstract

The widespread occurrence of carbapenem-resistant organisms has garnered significant public attention. Arthropods, including flies, are important vectors of multidrug-resistant bacteria. In this study, we reported the simultaneous carriage of four carbapenem-resistant isolates from different species, namely, Escherichia coli( E. coli), Providencia manganoxydans( P. manganoxydan), Myroides odoratimimus( M. odoratimimus) and Proteus mirabilis( P. mirabilis), from a single fly in China. These isolates were characterized through antimicrobial susceptibility testing, conjugation assays, whole-genome sequencing, and bioinformatics analysis. M. odoratimimusshowed intrinsic resistance to carbapenems. The mechanisms of carbapenem resistance in E. coli, P. manganoxydans, and P. mirabiliswere due to the production of NDM-5, NDM-1 and NDM-1, respectively. Genetic context of the bla _NDM genes in these three isolates varied. The bla _NDM-5 gene in E. coliwas located on an IncHI2/HI2A multidrug-resistant plasmid, which was conjugatively transferable. The bla _NDM-1 gene in P. mirabilisresided on the pPM14-NDM_123k-like nonconjugative plasmid. The bla _NDM-1 gene in P. manganoxydanswas found in a nonconjugatively transferable, multidrug-resistant region. The results of this study enhance our understanding of the dissemination of carbapenem-resistant organisms and suggest the need for a more comprehensive approach to antibiotic resistance research encompassing humans, animals, and the environment.

Keywords

Fly / Carbapenem-resistant organism / Whole-genome sequencing / Phenotypic characterization / bla _NDM

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Hanyu Wang, Hongwei Zhou, Gongxiang Chen, Ning Dong. Isolation of four carbapenem-resistant gram-negative species from a single fly. Animal Disease, 2024, 4(1): 4 https://doi.org/10.1186/s44149-024-00110-5

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