UV, five wavelengths fusion and electrochemical fingerprints combined with antioxidant activity for quality control of antiviral mixture

Abstract

Aiming to ensure the consistency of quality control of Traditional Chinese Medicines (TCMs), a combination method of high-performance liquid chromatography (HPLC), ultraviolet (UV), electrochemical (EC) was developed in this study to comprehensively evaluate the quality of Antiviral Mixture (AM), and Comprehensive Linear Quantification Fingerprint Method(CLQFM) was used to process the data. Quantitative analysis of three active substances in TCM was conducted. A five- wavelength fusion fingerprint (FWFF) was developed, using second-order derivatives of UV spectral data to differentiate sample levels effectively. The combination of HPLC and UV spectrophotometry, along with electrochemical fingerprinting (ECFP), successfully evaluated total active substances. Ultimately, a multidimensional profiling analytical system for TCM was developed.

Keywords

TCM / antiviral mixture / five-wavelength fusion fingerprint (FWFF) / Comprehensive Linear Quantification Fingerprint Method (CLQFM) / quantization fingerprint / antioxidant activity profilling

Conflict of interest

No potential conflict of interest was reported by the authors.

Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81573586).

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