Cultivation properties of human embryonic stem cells in the absence of serum and feeder layer

E. S. Philonenko; A. N. Kamnev; V. G. Zautorov; M. V. Shutova; L. V. Tskhovrebova; A. N. Bogomazova; S. L. Kiselev; M. A. Lagarkova

Genes & Cells ›› 2008, Vol. 3 ›› Issue (3) :31 -33.

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Cultivation properties of human embryonic stem cells in the absence of serum and feeder layer

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Abstract

Cultivation conditions for five human embryonic stem cell lines (hESM01, hESM02, hESM03, hESM04, HES-9) were switched from serum replacement containing medium with mouse embryonic fibroblasts as a feeder to two commercialy available media (mTESR1 by StemCell Technologies, Canada and StemPro by lnvitrogen, USA). Cell lines were maintained in these media for more than 20 passages. Matrigel (BD Biosciences, USA) was used as a substrate. hESCs cultivated in defined media and without animal derived components retain their pluripotency and differentiation ability. Changes in morphology of feeder-free hESC colonies were observed. Feeder-free hESC cultivation gives possibility to get larger amount of cells due to increase of proliferative activity.

Keywords

human embryonic stem cells (hESCs) / cultivation / pluripotency

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E. S. Philonenko, A. N. Kamnev, V. G. Zautorov, M. V. Shutova, L. V. Tskhovrebova, A. N. Bogomazova, S. L. Kiselev, M. A. Lagarkova. Cultivation properties of human embryonic stem cells in the absence of serum and feeder layer. Genes & Cells, 2008, 3(3): 31-33 DOI:

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