Human fetal liver and spleen (Cell composition)

Abstract

Cell composition has been studied in hepatic reprints of 76 human fetuses between 6-27 weeks of development. Erythropoiesis predominates during the whole period of hepatic hemopoiesis. During 6-7 weeks primary erythroblasts make 25% of all erythroid cells, and by the 22d-27^th weeks their content does not even reach 1,5%. Content of neutrophilic leucocytes is 1 - 1,5%. Nondifferentiated blast cells make 2,9±0,5 - 5,1±0,1%. Their greatest amount is observed between 6-8 and 18 and more weeks. The cell composition of the fetal spleen has been studied in 10 fetuses 19-25-week-old. Lymphoid forms make 85% of all cells of the organ’s hemopoietic tissue, of them 2,9±0,4% are lymphoblasts, 0,3±0,1% - nondifferentiated blasts. The investigations studying colonyforming properties of the hematopoietic cells confirmed that the highest content of myeloid precursors in the fetal liver and spleen is noted between 9-21 weeks of age. Therefore, it is possible to expect the greatest effect of these organ's transplantation during this time.

Keywords

fetal liver / fetal spleen / cell composition

References

[1] Kelemen Е. Recovery from chronic idiopathic bone marrow aplasia of a young mother after intravenous injections of unprocessed cells from the liver [and yolk sac) of her 22 mm CR-length embryo. A preliminary report. Scand. J. Haematol. 1973a; 10 (2): 305-8.

[2] Touraine I.L. Transplantation of stem cells from fully mismatched donors. Bone marrow transplant. 2007; 39 suppl 1: 42-3.

[3] Kansal V., Sood S.K., Barta A.K. etal. Fetallivertransplantationin aplastic anemia. Acta Haematol. 1979; 62 [3): 128-36.

[4] Fukuda T. Fetal hemopoiesis II. Electron microscopic studies on human hepatic hemopoiesis. Virchows Arch. B. Cell. Pathol. 1974; 16[3): 249-70.

[5] Fukuda T. Ultrastructure of fetal hemopoiesis. Acta Haematol. 1978; 41(6): 1204-13.

[6] Tarasov A.I., Petrenko A.Yu.., Jones D.R.E., Grischenko V.l. Phenotypic analysis and colony - forming activity of cryopreserved hemopoietic cells in human fetal liver. Experimental Oncol. 2002; 24:180-3.

[7] Orkin S.H., Zon L.l. Hematopoiesis and stem cells: plasticity versus developmental heterogeneity. Nature immunol. 2002; 3[4): 323-8.

[8] Tavian M., Cortes F., Charbord P. et al. Emergence of the haematopoietic system in the human embryo and fetus. Haematologica. 1999; 84 Suppl EHA-4:1: 1-3.

[9] Яновский Д.Н. Руководство по клинической гематологии. Киев, УССР: Госмедиздат; 1962.

[10] Migliaccio G., Migliaccio A.R., Petti S. et al. Human embryonic hemopoiesis! Kinetics of progenitors and precursors underlying the yolk -sac ? liver transition. J. Clin. Invest. 1986; 78: 51-60.

[11] Приндул Г. Гемопоэз в желточном мешке. Гематол. и трансфузиол. 1999:43 [3): 14-5.

[12] Чертков И.Л., Дризе Н.И., Воробьев А.И. и соавт. Кроветворение. В кн.: Руководство по гематологии, [ред. А.И.Воробьев). М., 2002; 1: 28-43.

[13] Kelemen Е., Calvo W., Fliedner T.M. Atlas of human hemopoietic development. Berlin: Springer Verlag; 1979.

[14] Ichikawa H. Differentiation of red pulp and evaluation of hemopoietic role of human prenatal spleen. Arch. Histol. Jap. 1985; 48[2): 183-97.

[15] Oguro M., Koizumi F., Sasagawa S. Blood cells in the peripheral blood and hematopoietic organs of the human fetus during hepatic hematopoiesis. Acta Haematol. 1978; 41[6): 1231-41.

[16] Герасимова Л.П. Колониеобразующая способность различных кроветворных и лимфоидных органов в процессе эмбрио- и фетогенеза человека. Пробл. гематол. 1980; 5: 45-8.

[17] Глузман Д.Ф. Диагностическая цитохимия гемобластозов. Киев: Наукова думка; 1978.

[18] Козинец Г.И., Шишканова З.Г., Сарычева Т.Г. и соавт. Клетки крови и костного мозга. Атлас. М. 2004.

[19] Kelemen Е., Petranyi Yu.lr., Puskas Е. Haemopoietic cell clusters too immature to permit classification in early human embryonic liver. A preliminary report. Haematologia 1973b; 3-4: 419-20.

[20] Kelemen E., Light microscopy of scattered unprocessed hemopoietic precursor cellsin liver smears of a 6,35 mm crown rump length human embryo. Exp. Hematol. 1974; 2(3): 118-27.

[21] Глузман Д.Ф., Бебешко В.Г., Надгорная B.A. и др. Эмбриональное кроветворение и гемобластозы у детей. Киев: Наукова думка; 1988.

[22] Алексеев И.В., Замараева И.В., Владимирская Е.Б., Курмашева Н.А. Развитие системы кроветворения у плода человека. Гематол. и трансфузиол. 1995; 4[5): 26-9.

[23] Петренко Ю.А. Иммунорегуляторные свойства клеток фетальной печени человека. Клеточная трансплантология и тканевая инженерия. 2007; ||[3): 57-61.

[24] Ярилин А.А. Основы иммунологии. М.: Медицина; 1999.

[25] Golfier F., Barcena А., Cruz J. et al. Mid-trimester fetal livers аге a rich source of CD34+/++ cells for transplantation. Bone Marrow Transplant. 1999; 24: 451-61.

[26] Раков A.A., Блинова T.C., Бубнов A.H. Коммитированные грануломоноцитарные предшественники в эмбриональных органах человека. Цитология 1981,23(4): 427-31

[27] Grischenko VI., Tarasov A.I., Rudenko S.V., Petrenko A.Yu. The sensitivity of human fetal liver cells of 7-12 weeks of gestation to programmed freesing and cyclic freeze-thawing. Probl. Cryobil. 2000; 4: 37-44.

[28] Раков A.A., Блинова T.C., Афанасьев Б.В. и др. Особенности миелопоэза в эмбриональной печени человека. Бюлл. экспер. биол. 1982; 94 [11): 92-4.

[29] Touraine J.L., Raudrant D., Laplace S. Transplantation of hemopoietic cells from the fetal liver to treat patient with congenital diseases postnatally or prenatally. Transplant Proc. 1997; 29: 712-3.

[30] Touraine J.L. Perinatal fetal-cell and gene therapy. Int. J. Immunopharmacol. 2000; 22:1033-40.

[31] Каргин В.Д., Абдулкадыров K.M. Применение трансплантаций эмбриональных гемопоэтических и лимфоидных органов и клеток при лечении заболеваний системы крови. Гематол. и трансфузиол. 1983; 7: 16-22.

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