Guided growth and migration of bone marrow stromal cells in culture in vitro

Abstract

Using a simple model in vitro the capability of rabbit bone marrow stromal cells to respond to chemotaxic stimulus by guiding migration and growth of the colony was substantiated. As a source of chemotaxic stimulus vital and devitalized laminar bone tissue fragments autogenic to cells culture were used. It has been stated that under cellular culture conditions stromal cells respond largely to vital tissue signals which might have been due to biological active substances produced by cells of a bone fragment. Obscure response of culture to devitalized bone tissue presence may have been due to bone tissue properties alteration after devitalization, in particular, alterations of intracellular matrix proteins.

Possible influence of different factors to cells culture migration has been discussed.

Keywords

stromal cells / chemotaxis / cells migration / bone tissue

References

[1] Денисов-Никольский Ю.И., Миронов С.П., Омельяненко Н.П., Матвейчук И.В. Актуальные проблемы теоретической и клинической остеоартрологии. М.: ОАО Типография «Новости» 2005: 336.

[2] Деев Р.В. Посттравматическая регенерация костной ткани при трансплантации культуры костномозговых стромальных клеток (экспериментальное исследование). Дис. .канд. мед. наук., СПб.; 2006: 175.

[3] Деев Р.В., Цупкина Н.В., Сериков В.Б. и др. Участие трансфузированных клеток костного мозга в репаративном остеогистогенезе. Цитология 2005; 46(9): 755-9.

[4] Dominici M., Pritchard C., Garlits J.E. et al. Hematopoietic cells and osteoblasts are derived from a common marrow progenitor after bone marrow transplantation. Proc. Natl. Acad. Sci. USA 2004; 101(32): 11761-6.

[5] Деев Р.В., Цупкина Н.В., Сергеев В.С. и др. Особенности физиологического и репаративного остеогенеза после трансфузии ядросодержащих клеток костного мозга. Клеточная трансплантология и тканевая инженерия 2006; 3(5): 54-8.

[6] Horwitz E.M., Prockop D.J., Gordon P.L. et al. Clinical responses to bone marrow transplantation in children with severe osteogenesis imperfecta. Blood 2001; 97(5): 1227-31.

[7] Horwitz E.M., Gordon P.L., Koo W.K. et al. Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: Implications for cell therapy of bone. Proc. Natl. Acad. Sci. USA 2002; 99(13): 8932-7.

[8] Миронов А.А., Комиссарчик Я.В., Миронов В.А. Методы электронной микроскопии в биологии и медицине: метод. руководство. СПб.: Наука 1994: 400.

[9] Илизаров Г.А., Палиенко Л.А., Шрейнер А.А., Богомяков В.С. Динамика численности костномозговых клеток, образующих колонии фибробластов в культуре, и её связь с активностью остеогенеза при репаративной регенерации в условиях удлинения конечности. Онтогенез 1983; 14(6): 617-23.

[10] Гололобов В.Г., Деев Р.В., Николаенко Н.С. и др. Характеристика культуры пластинчатой костной ткани in vitro. Морфология 2004; 125: 64-8.

[11] Kortesidis A., Zannettino A., Isenmann S. et al. Stromal-derived factor-1 promotes the growth, survival, and development of human bone marrow stromal stem cells. Blood 2005; 105(10): 3793-801.

[12] Neiva K., Sun Y.X., Taichman R.S. The role of osteoblasts in regulating hematopoietic stem cell activity and tumor metastasis. Braz. J. Med. Biol. Res. 2005; 38(10): 1449-54.

[13] Jung Y., Wang J., Schneider A. et al. Regulation of SDF-1 (CXCL12) production by osteoblasts; a possible mechanism for stem cell homing. Bone 2006; 38(4): 497-508.

[14] Lisignoli G., Toneguzzi S., Piacentini A. CXCL12 (SDF-1) and CXCL13 (BCA-1) chemokines significantly induce proliferation and collagen type I expression in osteoblasts from osteoarthritis patients. J. Cell Physiol. 2006; 206(1): 78-85.

[15] Lee D.H., Park B.J., Lee M.S. et al. Chemotactic Migration of Human Mesenchymal Stem Cells and MC3T3-E1 Osteoblast-Like Cells Induced by COS-7 Cell Line Expressing rhBMP-7. Tissue Eng. 2006; Epub. ahead of print.

[16] Fiedler J., Roderer G., Gunther K.P., Brenner R.E. BMP-2, BMP-4, and PDGF-bb stimulate chemotactic migration of primary human mesenchymal progenitor cells. J. Cell Biochem. 2002; 87(3): 305-12.

[17] Gonnerman K.N., Brown L.S., Chu T.M. Effects of growth factors on cell migration and alkaline phosphatase release. Biomed. Sci. Instrum. 2006; 42: 60-5.

[18] Makhijani N.S., Bischoff D.S., Yamaguchi D.T. Regulation of proliferation and migration in retinoic acid treated C3H10T1/2 cells by TGF-beta isoforms. J. Cell Physiol. 2005; 202(1): 304-13.

[19] Ma Z., Gao C., Gong Y., Shen J. Cartilage tissue engineering PLLA scaffold with surface immobilized collagen and basic fibroblast growth factor. Biomaterials 2005; 26(11): 1253-9.

[20] Karadag A., Fisher L.W. Bone sialoprotein enhances migration of bone marrow stromal cells through matrices by bridging MMP-2 to alpha(v)beta3-integrin. J. Bone Miner. Res. 2006; 21(10): 1627-36.

[21] Fiedler J., Roderer G., Gunther K.P., Brenner R.E. BMP-2, BMP-4, and PDGF-bb stimulate chemotactic migration of primary human mesenchymal progenitor cells. J. Cell Biochem. 2002; 87(3): 305-12.

[22] Fiedler J., Etzel N., Brenner R.E. To go or not to go: Migration of human mesenchymal progenitor cells stimulated by isoforms of PDGF. J. Cell Biochem. 2004; 93(5): 990-8.

[23] Celotti F., Colciago A., Negri-Cesi P. et al. Effect of platelet-rich plasma on migration and proliferation of SaOS-2 osteoblasts: role of platelet-derived growth factor and transforming growth factor-beta. Wound Repair Regen. 2006; 14(2): 195-202.

[24] Fiedler J., Leucht F., Waltenberger J. VEGF-A and PlGF-1 stimulate chemotactic migration of human mesenchymal progenitor cells. Biochem. Biophys. Res. Commun. 2005; 334(2): 561-8.

[25] Швед Ю.А., Кухарева Л.В., Блинова М.И. и др. Влияние различных форм коллагеновых субстратов, прикрепленных к полимерной подложке, на рост и пролиферацию клеток кожи в культуре. Цитология 2006; 48(9): 810-1.

[26] Suh H., Hwang Y.S., Lee J.E. et al. Behavior of osteoblasts on a type I atelocollagen grafted ozone oxidized poly L-lactic acid membrane. Biomaterials 2001; 22(3): 219-30.

RIGHTS & PERMISSIONS

Eco-Vector