Gene expression under control of regulatory elements of thermal shock gene of drozophyla in transgenic larvae germ of loach Misgurnus fossilis L.

Abstract

The efficiency of transient expression of lacZ and gfp genesreporters under control of two different promoter elements of drozophyla hsp70 thermal shochk gene in transgeneic larva of Fo generation loach Misgurnus fossilis L. were studied. The expression of genes in early larva and in the prelarval stage was mosaic and depended upon the amount of genetic material introduced.

At the same time gene expression did not depend upon external tempreture increase, i.e. it appeared without thermal shock.

In 2-3 day larva the efficiency of transient expression was maximal and reached 100% in separate experimental series. Thus, regulatory elements of drozophula thermal shock gene are capable to provide constitutional expression of gene-reporter in loach larva.

Keywords

gene expression / thermal shock proteins

References

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