Pathomorphological estimation of dimethyl selenium and dimethyl sulfoxide efficacy for the treatment of wounds in the experiment

Abstract

In the experiment on the wound model in rats it was studied the clinical efficacy of the drug composition with dimethyl selenium. Experimental studies were carried out on white outbred male rats, which under ether anesthesia on the surface of the back after depilation and alcohol lubrication excised skin up to 4 cm in diameter to underlying fascia. Animals in the first group as a means to stimulate the reparative processes of the skin wounds were treated with the drug composition with dimethyl selenium, animals of the second group (comparative group) were treated with dimethyl sulfoxide, animals of the third group (control) did not receive local treatment and its wounds healed under aseptic bandage. Evaluation of the efficacy of dimethyl selenite and dimethyl sulfoxide was performed on the 1st and 3rd day of treatment with light microscopy of wound tissue biopsies painted with hematoxylin and eosin, and pikrophuksine by Van Gieson method. It was found that wounds in animals of the control group were characterized by necrosis edge, hemorrhage, expressed soft tissue edema and inflammatory infiltration with more disintegrating polymorphonuclear leukocytes. Local use of the drug composition with dimethyl selenium led to a significant reduction in severity of pathological manifestations of inflammation and accelerating repair. Dimethyl sulfoxide also has anti-inflammatory effect, but less pronounced than dimethyl selenite. It is shown that the use of selenium in the local treatment of wounds to reduce necrosis in the wound edges, accelerate proliferative processes in the granulation tissue and thereby contribute to faster and more complete healing of wounds.

Keywords

wound / treatment / selenium / dimethyl selenite / dimethyl sulfoxide / efficacy / tissue biopsy / light microscopy / experiment / rats

References

[1] Беляев, А.Н. Системная и региональная антиоксидантная терапия при осложненных формах диабетической стопы / А.Н. Беляев, А.Н. Рыгин, А.Н. Захватов // Хирургия. – 2007. – № 11. – С. 46–50.

[2] Глухов, А.А. Клинико-морфологическое обоснование применения гидропрессивной санации и поляризованного облучения при лечении ран мягких тканей в эксперименте / А.А. Глухов, Н.Т. Алексеева, А.В. Лобцов // Вестн. эксперимент. и клин. хирургии. – 2010. – Т. 3, № 2. – С. 133–145.

[3] Костяков, Д.В. Современные возможности выбора патогенетически обоснованных методик лечения укушенных ран / Д.В. Костяков, Е.В. Зиновьев // Вестн. Росс. воен.-мед. академии. – 2015. – № 2 (54). – С. 235–240.

[4] Лиханов, И.Д. Влияние биорегулирующей терапии на иммунитет и гемостаз при гнойной хирургической инфекции / И.Д. Лиханов, М.Н. Цыбиков, Б.И. Кузник // Бюллетень ВСНЦ СО РАМН. – 2007. – № 5. – С. 128.

[5] Патент № 2369395 С1, Российская Федерация, МПК А61К 33/04. Средство для стимуляции репаративных процессов в ране / Е.В. Намоконов [и др.]; заявитель и патентообладатель ГОУ ВПО Читинская государственная медицинская академия Федерального агентства по здравоохранению и социальному развитию. – Заявка № 2008103046/15 от 28.01.2008. – Опубл. 10.10.2009. – Бюл. № 28. – 9 с.

[6] Привольнов, В.В. Выбор препарата для местного лечения инфицированных ран / В.В. Привольнов // Раны и раневая инфекция. Журн. им. проф. Б.М. Костюченок. – 2015. – № 1. – С. 13–22.

[7] Соловьева, А.Г. Роль оксида азота в процессах свободнорадикального окисления / А.Г. Соловьева [и др.] // Вест. Росс. воен.-мед. акад. – 2016. – № 1 (53). – С. 228–233.

[8] Хельсинкская декларация. Всемирная медицинская ассоциация. – М., 2001. – 52 с.

[9] Blueman, D. The use of larval therapy to reduce the bacterial load in chronic wounds / D. Bluesman, C. Bluefield // J. Wound Care. – 2012. – Vol. 21, № 5. – Р. 244–253.

[10] Murphy, P.S. Advances in wound healing: a review of current wound healing products / P.S. Murphy, G.R.D. Evans // Plats Surge Int. – 2012. – № 1. – Р. 190–199.

[11] Tran, P.L. A novel organo-selenium bandage that inhibits biofilm development in a wound by gram-positive and gram-negative wound pathogens // P.L. Tran [et al.] // Antibiotics. – 2014. – Vol. 3. – P. 435–449.

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