Evaluation of virucidal properties of the disinfectant agent based on polyhexamethyleneguanidine hydrochloride and alkyldimethylbenzylammonium chloride for the prevention of infections associated with health care

Abstract

The results of experimental studies to assess virucidal action of the disinfectant on the basis of polyhexamethyleneguanidine hydrochloride and alkyldimethylbenzylammonium chloride are discussed. To assess the virucidal properties of the test substance was used the concentrate of norovirus obtained from a pool of human faeces, of the hospitalized with acute gastroenteritis. Concentrate of norovirus has received by multi-stage purification using centrifugation and membrane filtration on filters of nitrocellulose, in a tangential flow. Initially, the presence of particles of norovirus in the preparation and the absence of a concomitant viral infection was confirmed using transmission electron microscopy using standard techniques of identification by characteristic morphological features and molecular biological confirmation. High virucidal activity of the drug was revealed. We can assume that a charged polycation (polyhexamethyleneguanidine hydrochloride and alkyldimethylbenzylammonium chloride) electrostatically interacts with the particles of norovirus. The interaction starts with the outer negatively charged P-domains, then S covers the hydrophobic domains, which leads to dissociation of the capsid and thus to the destruction of viral particles. RNA of norovirus in the absence of the receptor protein is not capable to infect more than that, it also breaks down and inactivates components of the disinfectant. Furthermore, the authors investigated the spectrum of the dominant circulating strains of microorganisms in a medical hospital. For the rotation of disinfectants in hospitals associated with medical care for the prevention of infections the polyhexamethyleneguanidine hydrochloride and alkyldimethylbenzylammonium chloride has been used. In a multidisciplinary hospital the above-mentioned disinfectant was applied, and positive results were obtained, testifying to the effectiveness of the disinfectant of polyhexamethyleneguanidine hydrochloride and alkyldimethylbenzylammonium chloride

Keywords

infections associated with health care / polyhexamethyleneguanidine hydrochloride / alkyldimethylbenzylammonium chloride / transmission electron microscopy / polymerase chain reaction in real time / membrane filtration / virucidal activity

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