Changing immunity and apoptosis in animals with carcinosarcoma Walker 256 after exposure to whole body hyperthermia (43,5°C)

Abstract

The dynamics of immune parameters changes and the apoptosis in animals with carcinosarcoma Walker 256 after exposure to whole body hyperthermia (43,5°C) is analyzed. It was found that the features of immunomodulatory and apoptosis modulatory effects of hyperthermia (43,5°C) in animals with tumors are as follows: the effect of hyperthermia is manifested by increasing number of of lymphocytes (CD3+, CD4+, CD8+ and CD20+) on day 7 and decreasing to 14 th day of the experiment. Against the backdrop of a temporary increase of lymphocytes Th 1-type notes a persistent increase of interferon-γ, which is a reflection of the activity of the cellular immune reactions of the body in response to hyperthermia. Furthermore, it was found the increasing of expression levels of pro-apoptotic protein Bad, Bax and decreasing of level of expression of the protein Bcl-2 anti-apoptotic in posthyperthermal period. For example, protein expression indicators in Bad carcinosarcoma Walker 256 cells at the 7th and 14th day posthyperthermal period were significantly (p<0,05) higher, respectively, 2,05 and 2,15 times as compared with the initial state. The expression of Bax protein level was also higher than the corresponding figure before the session of hyperthermia (43,5°C), on the 7th and 14th day in 3,52 and 1,69 times, respectively (p<0,05). We assume that hyperthermia as the method that is not related to the direct thermal damage to the tumor tissue is capable to provide immunomodulatory and apoptosis modulatory effects.

Keywords

Walker carcinosarcoma 256 / immunity / apoptosis / total hyperthermia / immunomodulatory and apoptosis modulatory effects of hyperthermia / posthyperthermal period / pro-apoptotic protein / tumor-bearing animals

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