EXPERIMENTAL SUBSTANTIATION OF THE IMMUNOSTIMULATING EFFECT OF BRANCHED-CHAIN AMINO ACIDS UNDER STRESS

E N Trushina; A N Timonin; N A Ryger; O K Mustafina; V D Kuznetsov

Medical academic journal ›› 2019, Vol. 19 ›› Issue (1S) :182 -184.

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EXPERIMENTAL SUBSTANTIATION OF THE IMMUNOSTIMULATING EFFECT OF BRANCHED-CHAIN AMINO ACIDS UNDER STRESS

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Abstract

The aim is to study the effect of branched-chain amino acid (BCAA) on cytokine synthesis in male Wistar rats on the background of cyclic starvation. A study of 2 months duration was performed on 60 rats, which were divided into 3 groups (20 rats in each group): rats of the control group (K-group) received standardized balanced laboratory feed for rodents ad libitum. The animals of the 1st experimental group (O-1 group) were fed according to the following scheme: the first day - feed ad libitum, the second and the third day - fasting, the rats of the 2nd experimental group (O-2 group) were given feed according to the same scheme, but with the addition of BCAA at a dosage of 70 mg/kg body weight per day. In the serum of rats, cytokine levels were determined: GM-CSF, INF-γ, IL-10, IL-12p70, IL-13, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL- 6, TNF-α on the Luminex 200 multiplex analyzer (Luminex Corporation, USA). Statistical processing of the results was performed using the IBM SPSS Statistics Version 20.0 application package (“IBM”, USA). As a result of the study, it was established that the consumption of BCAA by rats for 2 months on the background of cyclic starvation causes a decrease in the level of IL-10 and an increase in serum levels of IL-1a and IL-2 due to the activation of signaling pathways that regulate switching of the Th2 immune response to the predominant production of Th1 cytokines.

Keywords

branched chain amino acids (BCAA) / cyclic starvation / cytokines

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E N Trushina, A N Timonin, N A Ryger, O K Mustafina, V D Kuznetsov. EXPERIMENTAL SUBSTANTIATION OF THE IMMUNOSTIMULATING EFFECT OF BRANCHED-CHAIN AMINO ACIDS UNDER STRESS. Medical academic journal, 2019, 19(1S): 182-184 DOI:

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