THE CORTICOSTATIC EFFECT OF HUMAN LACTOFERRIN DEPENDS ON THE AMINO ACID COMPOSITION OF THE N-TERMINUS OF THE MOLECULE

G M Aleshina

Medical academic journal ›› 2019, Vol. 19 ›› Issue (1S) :159 -160.

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THE CORTICOSTATIC EFFECT OF HUMAN LACTOFERRIN DEPENDS ON THE AMINO ACID COMPOSITION OF THE N-TERMINUS OF THE MOLECULE

G M Aleshina

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Abstract

The effect of various structural variants of human lactoferrin on stress-induced changes in the corticosterone level in the blood in rats was studied. A model of combined emotional and physical stress - swimming in cold water (1-4 °C) for 2 minutes. The level of corticosterone in plasma was determined by enzyme immunoassay. We have previously found that preventive intraperitoneal administration of native lactoferrin reduces the stress-induced increase in corticosterone concentration in the blood of rats 30 minutes after stress. This work shows that transgenic human lactoferrin, which lacks four arginine residues at the N-terminus, does not have this effect. The obtained results allow us to conclude about the key role of N-terminal amino acid residues in the implementation of the corticostatic activity of lactoferrin.

Keywords

lactoferrin / corticosterone / stress

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G M Aleshina. THE CORTICOSTATIC EFFECT OF HUMAN LACTOFERRIN DEPENDS ON THE AMINO ACID COMPOSITION OF THE N-TERMINUS OF THE MOLECULE. Medical academic journal, 2019, 19(1S): 159-160 DOI:

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