EFFECTS OF KARMIZOLE DERIVATE ON THE EXPRESSION OF APO A-I GENE IN THE RAT’S HYPERLIPIDEMIA MODEL

Abstract

Azoles are the main antifungal drug class. The main mechanism of the azoles action is the intercalation in the sterol biosynthesis regulation. At the same time, the effect of the azole derivates on mammals is antiatherogenic. But there were no publication about connection between azole derivates effect on hyperlipidemia and expression of genes with antiatherogenic effects. In our work we used triton model of hyperlipodemia on rats to analyze the effect of carmizole injection on the expression of the main antiatherogenic genes and their regulators Apo A-I, HDL, LDL. We had four groups of rats: intact control group, triton control group, phenophibrate group and carmizole group. During a seven days we gave a per oral injections of carmizole for the carmizole group, phenophibrate (as a comparison drug) for phenophibrate group and 1% starch solution for triton control group. Liver tissue samples were used for RNA extraction and following RT-PCR (Real Time PCR) with primers for Apo A-I mRNA sequence. We have found, that Apo A-I mRNA level decreased in the triton control group to 17%, but restored up to 89% in the carmizole group. Carmizole derivate drug works like stimulator of Apo A-I gene expression. That increasing of the expression of antiatherogenic protein gene could me the base of the antiatherogenic effect of the carmizole derivate.

Keywords

azoles / carmizole / atherosclerosis / lipids / triglycerides / LDL / HDL

References

[1] Gsaller F, Hortschansky P, Furukawa T, et al. Sterol Biosynthesis and Azole Tolerance Is Governed by the Opposing Actions of SrbA and the CCAAT Binding Complex. Pathogens. https://doi.org/10.1371/journal.ppat.1006106.

[2] Fielding CJ, Fielding PE. Molecular physiology of reverse cholesterol transport. Journal of Lipid Research. 1995;36:211-218.

RIGHTS & PERMISSIONS

Lizunov A.V., Okunevich I.V., Shabanov P.D.