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Front Agric Chin    2011, Vol. 5 Issue (1) : 1-14     DOI: 10.1007/s11703-010-1070-2
RESEARCH ARTICLE |
Coordinate induction of antioxidant defense and glyoxalase system by exogenous proline and glycinebetaine is correlated with salt tolerance in mung bean
Mohammad Anwar HOSSAIN1,2, Mirza HASANUZZAMAN1,3, Masayuki FUJITA1()
1. Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan; 2. Department of Genetics & Plant Breeding, Bangladesh Agricultural University, Mymensingh- 2202, Bangladesh; 3. Department of Agronomy, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh
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Abstract

The purpose of this study was to assess the synergistic effects of exogenously applied proline and glycinebetaine (betaine) in antioxidant defense and methylglyoxal (MG) detoxification system in mung bean seedlings subjected to salt stress (200 mmol·L-1 NaCl, 48 h). Seven-day-old mung bean seedlings were exposed to salt stress after pre-treatment with proline or betaine. Salt stress caused a sharp increase in reduced glutathione (GSH) and oxidized glutathione (GSSG) content in leaves, while the GSH/GSSG ratio and ascorbate (AsA) content decreased significantly. The glutathione reductase (GR), glutathione peroxidase (GPX), glutathione S-transferase (GST) and glyoxalase II (Gly II) activities were increased in response to salt stress, while the monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), catalase (CAT) and glyoxalase I (Gly I) activities sharply decreased with an associated increase in hydrogen peroxide (H2O2) and lipid peroxidation level (MDA). Proline or betaine pre-treatment had little influence on non-enzymatic and enzymatic components as compared to those of the untreated control. However, proline or betaine pre-treated salt-stressed seedlings showed an increase in AsA, GSH content, GSH/GSSG ratio and maintained higher activities of APX, DHAR, GR, GST, GPX, CAT, Gly I and Gly II involved in ROS and MG detoxification system as compared to those of the untreated control and mostly also salt-stressed plants with a simultaneous decrease in GSSG content, H2O2 and MDA level. These results together with our previous results suggest that coordinate induction of antioxidant defense and glyoxalase system by proline and betaine rendered the plants tolerant to salinity-induced oxidative stress in a synergistic fashion.

Keywords salt stress      reactive oxygen species      antioxidant defense      methylglyoxal detoxification system      glycinebetaine      proline      mung bean     
Corresponding Authors: FUJITA Masayuki,Email:fujita@ag.kagawa-u.ac.jp   
Issue Date: 05 March 2011
 Cite this article:   
Mohammad Anwar HOSSAIN,Mirza HASANUZZAMAN,Masayuki FUJITA. Coordinate induction of antioxidant defense and glyoxalase system by exogenous proline and glycinebetaine is correlated with salt tolerance in mung bean[J]. Front Agric Chin, 2011, 5(1): 1-14.
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http://journal.hep.com.cn/fag/EN/Y2011/V5/I1/1
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Mohammad Anwar HOSSAIN
Mirza HASANUZZAMAN
Masayuki FUJITA
Fig.1  Schematic illustration of possible metabolic interaction of antioxidant defense and MG detoxification systems in plant cells
Note: Abbreviations are defined in the text.
Fig.2  Reduced ascorbate (AsA) contents in mung bean seedlings induced by proline and betaine under salt stress conditions
Note: Na, P, B, Na+ P and Na+ B indicates 200 mmol·L NaCl, 5 mmol·L proline, 5 mmol·L betaine, 200 mmol·L NaCl+ 5 mmol·L proline and 200 mmol·L NaCl+ 5 mmol·L betaine treatments, respectively. Mean (±SD) is calculated from three independent experiments. Bars with different letters are significantly different at <0.05.
Fig.3  Glutathione accumulation in mung bean seedlings induced by proline and betaine under salt stress conditions
Note: A is reduced glutathione (GSH), B is oxidized glutathione (GSSG) and C is GSH/GSSG ratio. Na, P, B, Na+ P and Na+ B indicate 200 mmol·L NaCl, 5 mmol·L proline, 5 mmol·L betaine, 200 mmol·L NaCl+ 5 mmol·L proline and 200 mmol·L NaCl+ 5 mmol·L betaine treatments, respectively. Mean (±SD) is calculated from three independent experiments. Bars with different letters are significantly different at <0.05.
Fig.4  Activities of APX (A), MDHAR (B), DHAR (C), GR (D), GPX (E), GST (F) and CAT (G) in mung bean seedlings induced by proline and betaine under salt stress conditions
Note: Na, P, B, Na+ P and Na+ B indicates 200 mmol·L NaCl, 5 mmol·L proline, 5 mmol·L betaine, 200 mmol·L NaCl+ 5 mmol·L proline and 200 mmol·L NaCl+ 5 mmol·L betaine treatments, respectively. Mean (±SD) is calculated from three independent experiments. Bars with different letters are significantly different at <0.05.
Fig.5  Activities of Gly I (A) and Gly II (B) in mung bean seedlings induced by proline and betaine under salt stress conditions
Note: Na, P, B, Na+ P and Na+ B indicate 200 mmol·L NaCl, 5 mmol·L proline, 5 mmol·L betaine, 200 mmol·L NaCl+ 5 mmol·L proline and 200 mmol·L NaCl+ 5 mmol·L betaine treatments, respectively. Mean (±SD) is calculated from three independent experiments. Bars with different letters are significantly different at <0.05.
Fig.6  Changes in HO concentration (A) and lipid peroxidation (represented by MDA) (B) level in mung bean seedlings induced by proline and betaine under salt stress conditions
Note: Na, P, B, Na+ P and Na+ B indicates 200 mmol·L NaCl, 5 mmol·L proline, 5 mmol·L betaine, 200 mmol·L NaCl+ 5 mmol·L proline and 200 mmol·L NaCl+ 5 mmol·L betaine treatments, respectively. Mean (±SD) is calculated from three independent experiments. Bars with different letters are significantly different at <0.05.
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