Vitamin C and E supplementation and high intensity interval training induced changes in lipid profile and haematological variables of young males

Abstract

High intensity interval training (HIIT) causes oxidative stress and haematological alteration. Present study was aimed to evaluate the effect of 8 weeks’ supplementation of vitamin C and E on HIIT induced changes in lipid profile parameters and haematological variables. Hundred six male adolescent players were randomly assigned into five age-matched groups, i.e., Control (no exercise+placebo), HIIT (placebo), HIIT ​+ ​vitamin-C (1 000 ​mg/day), HIIT ​+ ​vitamin-E 400 IU/day) and combined HIIT ​+ ​vitamin C and E. Morning and evening sessions (90 ​min) of HIIT included 4 phases (15 ​min each) with 3 sets (4 ​min each). Each 4 ​min HIIT set consisted of 2 ​min intense sprint workout (90%-95% of heart rate maximum [HR_max]) followed by 1 ​min active recovery (60%-70% HR_max) followed by 1 ​min of complete rest (1:1 work-rest ratio). Lipid profile parameters, haematological variables, endurance capacity and vertical jump were evaluated by standard protocols. Significant decrease in body weight, fat%, total cholesterol, triglyceride, Total Cholesterol/High Density Lipoprotein-Cholesterol and significant increase in High Density Lipoprotein-Cholesterol, maximal oxygen consumption, vertical jump were observed for all four intervention groups. White blood cell count, red blood cell count, haemoglobin percentage and haematocrit values were significantly decreased while platelet count and platelet-to-leukocyte ratio (PLR) ratio were increased significantly only for HIIT group. Blood level of tocopherol and ascorbic acid was significantly increased (values were within the normal range) in all the respective vitamin supplemented groups. Supplementation of vitamin C and E secures health protection with suppressed haemolysis and improved inflammatory blood variables with enhanced explosive leg strength and lipid profile parameters without any concomitant change in endurance capacity.

Keywords

Sprint interval training / Lipid profile / Haematological indices / Haemolysis / Athletes

Ethical approval

Written informed consent was obtained from each player and study protocol conformed to the ethical guidelines of the Declaration of Helsinki. Ethical clearance (Ref No. IHEC/AB/P82/2019) was obtained from the Institutional Human Ethical Committee (IHEC), Department of Physiology, University of Calcutta.

Conflict of interest

All authors have agreed to publish the present article and declare no conflict of interest.

Authors’ contributions

1. Conceptualization: Dr. Amit Bandyopadhyay and Dr.Swapan Kumar Dey.

2. Data collection: Surojit Sarkar.

3. Formal analysis: Surojit Sarkar, Amit Bandyopadhyay, Gouriprosad Datta.

4. Funding acquisition: Dr. Gouriprosad Datta and Dr.Swapan Kumar Dey.

5. Methodology: Surojit Sarkar and Dr.Swapan Kumar Dey.

6. Resources:Dr.Swapan Kumar Dey and Dr.GouriprosadDatta.

7. Supervision:Dr. Amit Bandyopadhyay, Dr. Swapan Kumar Dey and Dr. GouriprosadDatta.

8. Validation:Dr. Amit Bandyopadhyay, Dr. Swapan Kumar Dey and Dr.GouriprosadDatta.

9. Roles/Writing - original draft: Surojit Sarkar.

10. Writing review & editing: Dr. Amit Bandyopadhyay.

11. Confirmation of write up: All authors.

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