%A Anna Gortat,Mónica Sancho,Laura Mondragón,Àgel Messeguer,Enrique Pérez-Payá,Mar Orzáez %T Apaf1 inhibition promotes cell recovery from apoptosis %0 Journal Article %D 2015 %J Protein Cell %J Protein & Cell %@ 1674-800X %R 10.1007/s13238-015-0200-2 %P 833-843 %V 6 %N 11 %U {https://journal.hep.com.cn/pac/EN/10.1007/s13238-015-0200-2 %8 2015-11-04 %X

The protein apoptotic protease activating factor 1 (Apaf1) is the central component of the apoptosome, a multiprotein complex that activates procaspase-9 after cytochrome c release from the mitochondria in the intrinsic pathway of apoptosis. We have developed a vital method that allows fluorescence-activated cell sorting of cells at different stages of the apoptotic pathway and demonstrated that upon pharmacological inhibition of Apaf1, cells recover from doxorubicin- or hypoxia-induced early apoptosis to normal healthy cell. Inhibiting Apaf1 not only prevents procaspase-9 activation but delays massive mitochondrial damage allowing cell recovery.