%A Derong Xu,Guifeng Wei,Ping Lu,Jianjun Luo,Xiaomin Chen,Geir Skogerb?,Runsheng Chen %T Analysis of the p53/CEP-1 regulated non-coding transcriptome in C. elegans by an NSR-seq strategy %0 Journal Article %D 2014 %J Protein Cell %J Protein & Cell %@ 1674-800X %R 10.1007/s13238-014-0071-y %P 770-782 %V 5 %N 10 %U {https://journal.hep.com.cn/pac/EN/10.1007/s13238-014-0071-y %8 2014-10-24 %X

In recent years, large numbers of non-coding RNAs (ncRNAs) have been identified in C. elegans but their functions are still not well studied. In C. elegans, CEP-1 is the sole homolog of the p53 family of genes. In order to obtain transcription profiles of ncRNAs regulated by CEP-1 under normal and UV stressed conditions, we applied the ‘not-sorandom’ hexamers priming strategy to RNA sequencing in C. elegans, This NSR-seq strategy efficiently depleted rRNA transcripts from the samples and showed high technical replicability. We identified more than 1,000 ncRNAs whose apparent expression was repressed by CEP-1, while around 200 were activated. Around 40% of the CEP-1 activated ncRNAs promoters contain a putative CEP-1-binding site. CEP-1 regulated ncRNAs were frequently clustered and concentrated on the X chromosome. These results indicate that numerous ncRNAs are involved in CEP-1 transcriptional network and that these are especially enriched on the X chromosome in C. elegans.