LETTER

Phosphorylation of PLIN3 by AMPK promotes dispersion of lipid droplets during starvation

  • Jianxi Zhu 1,2 ,
  • Mingyang Xu 1 ,
  • Yi Liu 1 ,
  • Lisha Zhuang 1 ,
  • Kejun Ying 1 ,
  • Feng Liu 1 ,
  • Dan Liu 3 ,
  • Wenbin Ma , 1,2 ,
  • Zhou Songyang , 1,2,3
Expand
  • 1. MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
  • 2. Guangzhou Regenerative Medicine and Health-Guangdong Laboratory (GRMH-GDL), Guangzhou 510530, China
  • 3. Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA

Published date: 15 May 2019

Copyright

2018 The Author(s)

Cite this article

Jianxi Zhu , Mingyang Xu , Yi Liu , Lisha Zhuang , Kejun Ying , Feng Liu , Dan Liu , Wenbin Ma , Zhou Songyang . Phosphorylation of PLIN3 by AMPK promotes dispersion of lipid droplets during starvation[J]. Protein & Cell, 2019 , 10(5) : 382 -387 . DOI: 10.1007/s13238-018-0593-9

1
Bulankina AV, Deggerich A, Wenzel D, Mutenda K, Wittmann JG, Rudolph MG, Burger KN, Honing S (2009) TIP47 functions in the biogenesis of lipid droplets. J Cell Biol 185:641–655

DOI

2
Carling D, Aguan K, Woods A, Verhoeven AJ, Beri RK, Brennan CH, Sidebottom C, Davison MD, Scott J (1994) Mammalian AMPactivated protein kinase is homologous to yeast and plant protein kinases involved in the regulation of carbon metabolism. J Biol Chem 269:11442–11448

3
Dyck JR, Kudo N, Barr AJ, Davies SP, Hardie DG, Lopaschuk GD (1999) Phosphorylation control of cardiac acetyl-CoA carboxylase by cAMP-dependent protein kinase and 5’-AMP activated protein kinase. Eur J Biochem 262:184–190

DOI

4
Fraser SA, Choy SW, Pastor-Soler NM, Li H, Davies MR, Cook N, Katerelos M, Mount PF, Gleich K, McRae JL (2013) AMPK couples plasma renin to cellular metabolism by phosphorylation of ACC1. Am J Physiol Renal Physiol 305:F679–690

DOI

5
Herms A, Bosch M, Reddy BJ, Schieber NL, Fajardo A, Ruperez C, Fernandez-Vidal A, Ferguson C, Rentero C, Tebar F (2015) AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation. Nat Commun 6:7176

DOI

6
Hickenbottom SJ, Kimmel AR, Londos C, Hurley JH (2004) Structure of a lipid droplet protein; the PAT family member TIP47. Structure 12:1199–1207

DOI

7
Hocsak E, Racz B, Szabo A, Mester L, Rapolti E, Pozsgai E, Javor S, Bellyei S, Gallyas F Jr, Sumegi B (2010) TIP47 protects mitochondrial membrane integrity and inhibits oxidative-stressinduced cell death. FEBS Lett 584:2953–2960

DOI

8
Kaushik S, Cuervo AM (2016) AMPK-dependent phosphorylation of lipid droplet protein PLIN2 triggers its degradation by CMA. Autophagy 12:432–438

DOI

9
Kuo T, Chen TC, Lee RA, Nguyen NHT, Broughton AE, Zhang D, Wang JC (2017) Pik3r1 is required for glucocorticoid-induced perilipin 1 phosphorylation in lipid droplet for adipocyte lipolysis. Diabetes 66:1601–1610

DOI

10
Levin K, Daa Schroeder H, Alford FP, Beck-Nielsen H (2001) Morphometric documentation of abnormal intramyocellular fat storage and reduced glycogen in obese patients with type II diabetes. Diabetologia 44:824–833

DOI

11
Louche K, Badin PM, Montastier E, Laurens C, Bourlier V, de Glisezinski I, Thalamas C, Viguerie N, Langin D, Moro C (2013) Endurance exercise training up-regulates lipolytic proteins and reduces triglyceride content in skeletal muscle of obese subjects. J Clin Endocrinol Metab 98:4863–4871

DOI

12
Ohsaki Y, Cheng J, Fujita A, Tokumoto T, Fujimoto T (2006) Cytoplasmic lipid droplets are sites of convergence of proteasomal and autophagic degradation of apolipoprotein B. Mol Biol Cell 17:2674–2683

DOI

13
Patel S, Yang W, Kozusko K, Saudek V, Savage DB (2014) Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis. Proc Natl Acad Sci USA 111:9163–9168

DOI

14
Sanders MA, Madoux F, Mladenovic L, Zhang H, Ye X, Angrish M, Mottillo EP, Caruso JA, Halvorsen G, Roush WR (2015) Endogenous and synthetic ABHD5 ligands regulate ABHD5-perilipin interactions and lipolysis in fat and muscle. Cell Metab 22:851–860

DOI

15
Storey SM, McIntosh AL, Senthivinayagam S, Moon KC, Atshaves BP (2011) The phospholipid monolayer associated with perilipinenriched lipid droplets is a highly organized rigid membrane structure. Am J Physiol 301:E991–E1003

DOI

Outlines

/