Crystal structures of Bbp from Staphylococcus aureus reveal the ligand binding mechanism with Fibrinogen α

Abstract

Bone sialoprotein-binding protein (Bbp), a MSCRAMMs (Microbial Surface Components Recognizing Adhesive Matrix Molecules) family protein expressed on the surface of Staphylococcus aureus (S. aureus), mediates adherence to fibrinogen α (Fg α), a component in the extracellular matrix of the host cell and is important for infection and pathogenesis. In this study, we solved the crystal structures of apo-Bbp^273−598 and Bbp^273−598-Fg α^561−575 complex at a resolution of 2.03 Å and 1.45 Å, respectively. Apo-Bbp^273−598 contained the ligand binding region N2 and N3 domains, both of which followed a DE variant IgG fold characterized by an additional D1 strand in N2 domain and D1′ and D2′ strands in N3 domain. The peptide mapped to the Fg α^561−575 bond to Bbp^273−598 on the open groove between the N2 and N3 domains. Strikingly, the disordered C-terminus in the apo-form reorganized into a highly-ordered loop and a β-strand G′′ covering the ligand upon ligand binding. Bbp^{Ala298−Gly301} in the N2 domain of the Bbp^273−598-Fg α^561−575 complex, which is a loop in the apo-form, formed a short α-helix to interact tightly with the peptide. In addition, Bbp^{Ser547−Gln561} in the N3 domain moved toward the binding groove to make contact directly with the peptide, while Bbp^{Asp338−Gly355} and Bbp^{Thr365−Tyr387} in N2 domain shifted their configurations to stabilize the reorganized C-terminus mainly through strong hydrogen bonds. Altogether, our results revealed the molecular basis for Bbp-ligand interaction and advanced our understanding of S. aureus infection process.

Key words： bone sialoprotein-binding protein (Bbp); fibrinogen; serine-aspartate repeat (Sdr); Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMM); Staphylococcus aureus

References

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