Tcf7l1 promotes transcription of Kruppel-like factor 4 during Xenopus embryogenesis

Qing Cao, Yan Shen, Wei Zheng, Hao Liu, Chen Liu

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Journal of Biomedical Research ›› 2018, Vol. 32 ›› Issue (3) : 215-221. DOI: 10.7555/JBR.32.20170056
Original Article

Tcf7l1 promotes transcription of Kruppel-like factor 4 during Xenopus embryogenesis

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Abstract

Kruppel-like factor 4 (Klf4) is a zinc finger transcription factor and plays crucial roles in Xenopus embryogenesis. However, its regulation during embryogenesis is still unclear. Here, we report that Tcf7l1, a key downstream transducer of the Wnt signaling pathway, could promote Klf4 transcription and stimulate Klf4 promoter activity in early Xenopus embryos. Furthermore, cycloheximide treatment showed a direct effect on Klf4 transcription facilitated by Tcf7l1. Moreover, the dominant negative form of Tcf7l1 (dnTcf7l1), which lacks N-terminus of the β-catenin binding motif, could still activate Klf4 transcription, suggesting that this regulation is Wnt/β-catenin independent. Taken together, our results demonstrate that Tcf7l1 lies upstream of Klf4 to maintain its expression level during Xenopus embryogenesis.

Keywords

Kruppel-like factor 4 (Klf4) / Tcf7l1 / transcription regulation / Xenopus laevis

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Qing Cao, Yan Shen, Wei Zheng, Hao Liu, Chen Liu. Tcf7l1 promotes transcription of Kruppel-like factor 4 during Xenopus embryogenesis. Journal of Biomedical Research, 2018, 32(3): 215‒221 https://doi.org/10.7555/JBR.32.20170056

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Acknowledgment

We thank Model Animal Research Center of Nanjing University for their assistance in data collection. This work was supported by the Start-up Funding of Henan University of Science and Technology (13480027) to Q.C. and the Key Science Foundation of Nanjing Medical University(2015NJMUZD002) and the Natural Science Foundation of Higher Education Institutions of Jiangsu Province (16KJB-180020) and Natural Science Foundation of Jiangsu Province (BK20171053) and National Natural Science Funds of China (81702747) to C.L.

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2017 2018 by the Journal of Biomedical Research.
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