Distinctive roles of Rac1 and Rab29 in LRRK2 mediated membrane trafficking and neurite outgrowth

Abstract

Parkinson’s disease (PD) associated leucine-rich repeat kinase 2 (LRRK2) mutants have shown pathogenic effects on variety of subcellular processes.Two small GTPases Rac1 and Rab29 have been indicated as possible downstream effectors participating in LRRK2 signaling but their detail mechanisms remain unclear. In this study, we have used biochemical and cell biology approaches to address whether two GTPases interact with LRRK2 and hence function differently in LRRK2 mediated pathogenesis.Here we show thatRac1 and Rab29 specifically interact with LRRK2with higher affinity for Rab29and with different preference in functional domain binding. Mutant Rab29 but not Rac1 alters theendosome-to-TGN retrograde trafficking of a cargo protein cation-independent mannose-6-phosphate receptor (CI-M6PR) and its stability. On the other hand, overexpressedwild type Rab29 but not Rac1 rescue the altered retrograde membrane trafficking induced by the pathogenic mutant LRRK2^G2019S. Furthermore, both Rac1 and Rab29 can rescue the neurite shortening in differentiated SH-SY5Y cells induced by LRRK2^G2019S. Our study strongly suggests that Rac1 and Rab29 are involved in the distinct functions as downstream effectors in LRRK2 signaling pathways.

Keywords

Parkinson’s disease / LRRK2 / Rac1 / Rab29 / retrograde trafficking

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Acknowledgments

This work was supported by the National Nature Science Foundation of China (Grant No. 31371436 and No. 8157051134) and by the laboratory start-up grant from Nanjing Medical University to Y. Liu. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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2017 2017 by the Journal of Biomedical Research. All rights reserved