Molecular characterization of antimicrobial multi-drug resistance in non-typhoidal Salmonellae from chicken and clam in Mangalore, India
Yemisi Olukemi Adesiji, Santhosh Kogaluru Shivakumaraswamy, Vijaya Kumar Deekshit, Girisha Shivani Kallappa, Indrani Karunasagar
Molecular characterization of antimicrobial multi-drug resistance in non-typhoidal Salmonellae from chicken and clam in Mangalore, India
Salmonella enterica has been documented as one of the leading causes of salmonellosis throughout the world and is most commonly associated with the consumption of contaminated food products. Thus, this research was aimed at studying the antimicrobial susceptibility pattern and detection of quinolone resistance in Salmonella spp isolated from food of animal origin. Thirty-six Salmonella isolates comprising 8 from poultry and 28 from seafood (clams) were identified, serotyped and characterized for their antimicrobial susceptibility against 10 different antibiotics. Plasmid DNA was isolated from all the isolates by alkaline lysis, quinolone resistant non-typhoidal S. Weltevreden were examined for mutation in the DNA gyrase coding gene. Among the 36 Salmonella isolates, 20 were S. weltevreden (8 from poultry and 12 from seafood) and 16 were S. Typhimurium (from seafood). All the isolates showed multiple resistance to nalidixic acid, tetracycline, co-trimoxazole and nitrofurantoin, but, interestingly, the isolates were 100% susceptible to ampicillin, chloramphenicol and gentamicin. Resistant isolates from the study carried the genes responsible for resistance to respective antibiotics. The strain S130 isolated in the study showed single point mutation, Asp87Gly, at position 87 in quinolone resistance determining region. It revealed mutation in quinolone resistance determining region as a cause for quinolone resistance in non-typhoidal Salmonellae. The occurrence of genes accountable for plasmid mediated resistance to quinolones (viz., qnrA, qnrB and qnrS) in plasmid of non-typhoidal Salmonellae isolates provides evidence for plasmid mediated quinolone resistance.
mutation / multi-drug resistant / non-typhoidal Salmonellae / plasmid mediated quinolone resistance / quinolone resistance determining region
[1] |
Hohmann EL. Nontyphoidal salmonellosis[J]. Clin Infect Dis, 2001, 32(2): 263–269.
CrossRef
Pubmed
Google scholar
|
[2] |
Al-Dawodi R, Farraj MA, Essawi T. Antimicrobial resistance in non-typhi Salmonella enterica isolated from humans and poultry in Palestine[J]. J Infect Dev Ctries, 2012, 6(2): 132–136
Pubmed
|
[3] |
Rice DH, Hancock DD, Roozen PM,
CrossRef
Pubmed
Google scholar
|
[4] |
Han J, Gokulan K, Barnette D,
CrossRef
Pubmed
Google scholar
|
[5] |
Crump JA, Sjölund-Karlsson M, Gordon MA,
CrossRef
Pubmed
Google scholar
|
[6] |
Oliveira CM, Ribeiro AR, Santos LR,
CrossRef
Google scholar
|
[7] |
Guan X, Xue X, Liu Y,
CrossRef
Pubmed
Google scholar
|
[11] |
Randall LP, Coldham NG, Woodward MJ. Detection of mutations in Salmonella enterica gyrA, gyrB, parC and parE genes by denaturing high performance liquid chromatography (DHPLC) using standard HPLC instrumentation[J]. J Antimicrob Chemother, 2005, 56(4): 619–623.
CrossRef
Pubmed
Google scholar
|
[12] |
Haugum K, Aas L, Lindstedt BA. Effect of quinolone antibiotics and chemicals on mutation types in Salmonella enterica serovars Enteritidis, Hadar and Virchow[J]. Division for Infectious Diseases Control, Norwegian Institute of Public Health, N-0403 Oslo, Norway 2006; 1–5.
|
[13] |
Martínez-Martínez L, Pascual A, Jacoby GA. Quinolone resistance from a transferable plasmid[J]. Lancet, 1998, 351(9105): 797–799.
CrossRef
Pubmed
Google scholar
|
[14] |
Saha SK, Talukder SY, Islam M,
CrossRef
Pubmed
Google scholar
|
[15] |
Akinyemi KO, Bamiro BS, Coker AO. Salmonellosis in Lagos, Nigeria: incidence of Plasmodium falciparum-associated co-infection, patterns of antimicrobial resistance, and emergence of reduced susceptibility to fluoroquinolones[J]. J Health Popul Nutr, 2007, 25(3): 351–358
Pubmed
|
[16] |
Adesiji YO, Alli O, Adekanle M,
CrossRef
Google scholar
|
[17] |
Mensah P, Mwamakamba L, Kariuki S,
|
[18] |
Andrews WH, Hammack T. Salmonella. In Bacteriological Analytical Manual US Food and Drug Administration[J]. 2011. Available from: http://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/default.htm.
|
[19] |
MacFaddin JF. Biochemical tests for identification of medical bacteria[J], 3rd ed. Lippincott Williams & Wilkins, Philadelphia 2000.
|
[20] |
Rahn K, De Grandis SA, Clarke RC,
CrossRef
Pubmed
Google scholar
|
[21] |
Jones DD, Law R, Bej AK. Detection of Salmonella spp[J]. in oysters using polymerase chain reaction (PCR) and gene probes. J Food Sci, 1993, 58(6): 1191–1197
CrossRef
Google scholar
|
[22] |
Casin I, Breuil J, Darchis JP,
CrossRef
Pubmed
Google scholar
|
[23] |
Robicsek A, Strahilevitz J, Sahm DF,
CrossRef
Pubmed
Google scholar
|
[24] |
Ma M, Wang H, Yu Y,
CrossRef
Pubmed
Google scholar
|
[25] |
Aarestrup FM, Agerso Y, Gerner-Smidt P,
CrossRef
Pubmed
Google scholar
|
[26] |
Bauer AW, Kirby WMM, Sherris JC,
Pubmed
|
[27] |
Singh S, Yadav AS, Singh SM,
CrossRef
Google scholar
|
[28] |
Ausubel F, Brent R, Kingston RE,
|
[29] |
Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual[J]. Small Scale Preparation of Plasmid DNA: Alkaline Lysis Method. Cold Spring Harbor Laboratory Press, New York. 1989; 1.25–1.27.
|
[30] |
Hohmann EL. Nontyphoidal salmonellosis[J]. Clin Infect Dis, 2001, 32(2): 263–269.
CrossRef
Pubmed
Google scholar
|
[31] |
Falagas ME, Michalopoulos A. Polymyxins: old antibiotics are back[J]. Lancet, 2006, 367(9511): 633–634.
CrossRef
Pubmed
Google scholar
|
[32] |
Majumder S, Rahmatullah M. Efficacy of old antibiotics against commonly isolated bacterial isolates in a tertiary care hospital[J]. J Chem Pharm Res, 2015, 7(4): 1392–1396.
|
[33] |
Helms M, Vastrup P, Gerner-Smidt P,
CrossRef
Pubmed
Google scholar
|
[34] |
Weigel LM, Steward CD, Tenover FC. gyrA mutations associated with fluoroquinolone resistance in eight species of Enterobacteriaceae[J]. Antimicrob Agents Chemother, 1998, 42(10): 2661–2667
Pubmed
|
[35] |
Hakanen A, Kotilainen P, Huovinen P,
CrossRef
Pubmed
Google scholar
|
[36] |
Reyna F, Huesca M, González V,
CrossRef
Pubmed
Google scholar
|
[37] |
Giraud E, Baucheron S, Cloeckaert A. Resistance to fluoroquinolones in Salmonella: emerging mechanisms and resistance prevention strategies[J]. Microbes Infect, 2006, 8(7): 1937–1944.
CrossRef
Pubmed
Google scholar
|
[38] |
Hernández A, Sánchez MB, Martínez JL. Quinolone resistance: much more than predicted[J]. Front Microbiol, 2011, 2(22): 22.
Pubmed
|
/
〈 | 〉 |