Effects of epinephrine on angiogenesis-related gene expressions in cultured rat cardiomyocytes

Abstract

Epinephrine is often used for the treatment of patients with heart failure, low cardiac output and cardiac arrest. It can acutely improve hemodynamic parameters; however, it does not seem to improve longer term clinical outcomes. Therefore, we hypothesized that epinephrine may induce unfavorable changes in gene expression of cardiomyocyte. Thus, we investigated effects of epinephrine exposure on the mediation or modulation of gene expression of cultured cardiomyocytes at a genome-wide scale. Our investigation revealed that exposure of cardiomyocytes to epinephrine in an in vitro environment can up-regulate the expression of angiopoietin-2 gene (+2.1 times), and down-regulate the gene expression of neuregulin 1 (−3.7 times), plasminogen activator inhibitor-1 (−2.4 times) and SPARC-related modular calcium-binding protein-2 (−4.5 times). These changes suggest that epinephrine exposure may induce inhibition of angiogenesis-related gene expressions in cultured rat cardiomyocytes. The precise clinical significance of these changes in gene expression, which was induced by epinephrine exposure, warrants further experimental and clinical investigations.

Keywords

epinephrine / angiogenesis / gene expression / cardiomyocytes / angiopoietin-2 / neuregulin 1 / plasminogen activator inhibitor-1 / SPARC-related modular calcium-binding protein

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Acknowledgement

This study was supported by internal funding from the Department of Anesthesiology and Perioperative Medicine. A portion of our study results was presented as an abstract in the American Society of Anesthesiologists annual meeting on October 13^th, 2014 in New Orleans, Louisiana.

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