Specific chromatin states and m6A modifications are associated with mRNA mobility in planta

Acknowledgements

We thank Dr. Murat Iskar for his feedback, 2020 Marie-Curie fellowship 885864 to Y.D., National Natural Science Foundation of China 32372792 and the 2115 Talent Development Program of China Agricultural University to W.Z., and German Federal Ministry of Education and Research 031B1231B to V.V.U.

Author contribution

Y.D., V.V.U. and W.Z. initiated the project. Y.D. and V.V.U. performed analysis in Arabidopsis. X.L., Y.C., W.Z. and X.H. performed analysis in cucumber and pumpkin. Y.D. wrote the manuscript. A.B., V.V.U. and W.Z. revised the manuscript.

Data availability

All data generated for this study are included in the manuscript.

Conflict of interest statement

The authors declare no conflict of interest.

References

[1] Yang L, Perrera V, Saplaoura E. et al. M(5)C methylation guides systemic transport of messenger RNA over graft junctions in plants. Curr Biol. 2019; 29:2465-2476.e5

[2] Calderwood A, Kopriva S, Morris RJ. Transcript abundance explains mRNA mobility data in Arabidopsis thaliana. Plant Cell. 2016; 28:610-5

[3] Zhang D, Guo W, Wang T. et al. RNA 5-methylcytosine modifi-cation regulates vegetative development associated with H3K27 trimethylation in Arabidopsis. Adv Sci (Weinh). 2022; 10:e2204885

[4] Sequeira-Mendes J, Araguez I, Peiro R. et al. The functional topography of the Arabidopsis genome is organized in a reduced number of linear motifs of chromatin states. Plant Cell. 2014; 26: 2351-66

[5] Thieme CJ, Rojas-Triana M, Stecyk E. et al. Endogenous Arabidop-sis messenger RNAs transported to distant tissues. Nat Plants. 2015; 1:15025

[6] Charron JB, He H, Elling AA. et al. Dynamic landscapes of four histone modifications during deetiolation in Arabidopsis. Plant Cell. 2009; 21:3732-48

[7] Li Y, Brooks M, Yeoh-Wang J. et al. SDG8-mediated histone methylation and RNA processing function in the response to nitrate signaling. Plant Physiol. 2020; 182:215-27

[8] Huang H, Weng H, Zhou K. et al. Histone H3 trimethylation at lysine 36 guides m(6)a RNA modification co-transcriptionally. Nature. 2019; 567:414-9

[9] Luo GZ, MacQueen A, Zheng G. et al. Unique features of the m6A methylome in Arabidopsis thaliana. Nat Commun. 2014; 5:5630

[10] Loedige I, Baranovskii A, Mendonsa S. et al. mRNA stability and m(6)a are major determinants of subcellular mRNA localization in neurons. Mol Cell. 2023; 83:2709-2725.e10