CRISPR/Cas9 revolutionizes Macleaya cordata breeding: a leap in sanguinarine biosynthesis

Acknowledgements

This work was supported by Hunan Innovative Province Construction Special Fund Grant, Yuelu Mountain Seed Industry Innovation Project (2021NK1012) and the Hunan Provincial Natural Science Foundation of China (2023JJ40367, 2023JJ30341, 2021JJ20032).

Author contributions

M.S., P.H., and J.Z. conceived and designed the study. M.S. and P.H. wrote the manuscript. M.S. and L.Z. conducted the experimental work. R.S., X.Z., and W.L. participated in the results interpretation. All authors read and approved the final manuscript.

Data availability statement

Two genes from M. cordata, McPDS (GenBank accession number OR502428) and McSMT (OR502429), have been deposited in GenBank. Transcriptome raw data in this study have been deposited in the SRA database (https://www.ncbi.nlm.nih.gov/sra) under project number PRJNA1018289.

Conflict of interest

The authors declare no competing interests.

References

[1] Jessica W, Brett S, Shannon B. et al. Antibiotics and antibiotic resistance in agroecosystems: state of the science. J Environ Qual. 2016; 45:394-406

[2] Liu X, Liu Y, Huang P. et al. The genome of medicinal plant Macleaya cordata provides new insights into benzylisoquinoline alkaloids metabolism. Mol Plant. 2017; 10:975-89.

[3] Syombua ED, Zhang Z, Tripathi JN. et al. A CRISPR/Cas9-based genome-editing system for yam (Dioscorea spp.). Plant Biotechnol J. 2021; 19:645-7

[4] Zhang X, Xu G, Cheng C. et al. Establishment of an Agrobacterium-mediated genetic transformation and CRISPR/Cas9-mediated targeted mutagenesis in hemp (cannabis sativa L.). Plant Biotechnol J. 2021; 19:1979-87

[5] Du H, Zeng X, Zhao M. et al. Efficient targeted mutagenesis in soybean by TALENs and CRISPR/Cas9. J Biotechnol. 2016; 217:90-7

[6] Ma X, Zhang Q, Zhu Q. et al. A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Mol Plant. 2015; 8:1274-84

[7] Tu M, Fang J, Zhao R. et al. CRISPR/Cas9-mediated mutagenesis of VvbZIP36 promotes anthocyanin accumulation in grapevine (Vitis vinifera). Hortic Res. 2022; 9:uhac022

[8] Li J, Manghwar H, Sun L. et al. Whole genome sequencing reveals rare off-target mutations and considerable inherent genetic or/and somaclonal variations in CRISPR/Cas9-edited cotton plants. Plant Biotechnol J. 2019; 17:858-68

[9] Wang X, Tu M, Wang Y. et al. Whole-genome sequencing reveals rare off-target mutations in CRISPR/Cas9-edited grapevine. Hortic Res. 2021; 8:114

[10] Zhou Z, Tan H, Li Q. et al. CRISPR/Cas9-mediated efficient targeted mutagenesis of RAS in Salvia miltiorrhiza. Phytochemistry. 2018; 148:63-70