Efficient targeted mutagenesis in tetraploid Pogostemon cablin by the CRISPR/Cas9-mediated genomic editing system

Acknowledgements

The research was supported by the Research and Development Program in Key Areas of Guangdong Province (No. 2021B0707010010), the Scientific Innovation Strategy-Construction of High-level Academy of Agriculture Science (R2019PY-JX003), the Director’s Fund of Crop Research Institute of Guangdong Academy of Agricultural Sciences/Open Fund of Guangdong Provincial Key Laboratory of Crops Genetics and Improvement (202206), and the Special Fund for Introducing Scientific and Technological Talents of Guangdong Academy of Agricultural Sciences (R2020YJ-YB3003).

Author contributions

S.Q.X., J.H.W., and S.H.Y. designed and supervised the project, S.Q.X., F.Z., J.Y.L., and S.K.C. prepared the samples, S.Q.X., F.Z., and J.Y.L. performed the experiments, S.Q.X., F.P.L., and J.H.W. analyzed the data. S.Q.X. wrote the paper and S.Q.X., F.P.L., S.H.Y., and J.H.W. revised the paper. All authors read and approved the final manuscript.

Data availability

The data sets supporting the conclusions of this article are available in the Figshare database under the accession 10.6084/m9.figshare.24850635.

Conflict of interest

The authors declare no conflict of interest.

References

[1] Swamy MK, Sinniah UR. A comprehensive review on the phytochemical constituents and pharmacological activities of Pogostemon cablin Benth.: an aromatic medicinal plant of industrial importance. Molecules. 2015; 20:8521-47

[2] Isnaini N, Khairan K, Faradhilla M. et al. A study of essential oils from patchouli (Pogostemon cablin Benth.) and its potential as an antivirus agent to relieve symptoms of COVID-19. J Patchouli Essent Oil Prod. 2022; 1:27-35

[3] Ren JL, Zhang AH, Wang XJ. Traditional Chinese medicine for COVID-19 treatment. Pharmacol Res. 2020; 155:104743

[4] Chen XY, Gan BC, Sui C. et al. Resistance evaluation of 20 Pogostemon cablin germplasms to bacterial wilt. Acta Agric Boreali-Occident Sin. 2012; 21:170-4

[5] Sugimura Y, Kadotani N, Ueda Y. et al. Transgenic patchouli plants produced by Agrobacterium-mediated transformation. Plant Cell Tissue Organ Cult. 2005; 82:251-7

[6] Paul A, Bakshi S, Sahoo DP. et al. Agrobacterium-mediated genetic transformation of Pogostemon cablin (Blanco) Benth. using leaf explants: bactericidal effect of leaf extracts and counteracting strategies. Appl Biochem Biotechnol. 2012; 166:1871-95

[7] Shen YT, Li WY, Zeng Y. et al. Chromosome-level and haplotype-resolved genome provides insight into the tetraploid hybrid origin of patchouli. Nat Commun. 2022; 13:1-15

[8] Liu JX, Li T, Wang H. et al. CRISPR/Cas9-mediated precise targeted mutagenesis of phytoene desaturase in celery. Hortic Res. 2022; 9:uhac162

[9] Zhou Z, Tan H, Li Q. et al. CRISPR/Cas9-mediated efficient targeted mutagenesis of RAS in Salvia miltiorrhiza. Phytochemistry. 2018; 148:63-70

[10] Kishi-Kaboshi M, Aida R, Sasaki K. Parsley ubiquitin promoter displays higher activity than the CaMV 35S promoter and the chrysanthemum actin 2 promoter for productive, constitutive, and durable expression of a transgene in Chrysanthemum morifolium. Breed Sci. 2019; 69:536-44