Physiological and molecular bases of the boron deficiency response in tomatoes

Abstract

Boron is an essential microelement for plant growth. Tomato is one of the most cultivated fruits and vegetables in the world, and boron deficiency severely inhibits its yield and quality. However, the mechanism of tomato in response to boron deficiency remains largely unclear. Here, we investigated the physiological and molecular bases of the boron deficiency response in hydroponically grown tomato seedlings. Boron deficiency repressed the expression of genes associated with nitrogen metabolism, while it induced the expression of genes related to the pentose phosphate pathway, thereby altering carbon flow to provide energy for plants to cope with stress. Boron deficiency increased the accumulation of copper, manganese and iron, thereby maintaining chlorophyll content and photosynthetic efficiency at the early stage of stress. In addition, boron deficiency downregulated the expression of genes involved in cell wall organization and reduced the contents of pectin and cellulose in roots, ultimately retarding root growth. Furthermore, boron deficiency markedly altered phytohormone levels and signaling pathways in roots. The contents of jasmonic acid, jasmonoy1-L-isoleucine, trans-zeatin riboside, abscisic acid, salicylic acid, and SA glucoside were decreased; in contrast, the contents of isopentenyladenine riboside and ethylene precursor 1-aminocyclopropane-1-carboxylic acid were increased in the roots of boron-deficient tomato plants. These results collectively indicate that tomato roots reprogram carbon/nitrogen metabolism, alter cell wall components and modulate phytohormone pathways to survive boron deficiency. This study provides a theoretical basis for further elucidating the adaptive mechanism of tomato in response to boron deficiency.

Acknowledgements

This research was supported by the China National Natural Sciences Foundation (32070314) to J.X., the Science and Technology Innovation Fund project of Shanxi Agricultural University (2020BQ24) to P.Z., and the Basic Research Program of Shanxi Province (Free Exploration) (20210302124369) to L.S.

Author contributions

J.L.: investigation, data curation, formal analysis, visualization, writing-original draft, writing-review & editing. H.F.: investigation, visualization. L.J.: investigation, visualization. P.Z.: methodology, conceptualization. F.L.: methodology. L.S.: methodology. H.Y.: investigation. Q.S.: investigation. R.L.: investigation. W.L.: investigation. J.X.: Writing-review & editing, supervision, conceptualization, methodology, validation, supervision, project administration.

Data availability

The raw data of RNA-seq were archived at the SRA of NCBI under accession no. PRJNA910924.

Conflict of interest

The authors have no conflict of interest to declare.

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