SlMYB72 affects pollen development by regulating autophagy in tomato

Abstract

The formation and development of pollen are among the most critical processes for reproduction and genetic diversity in the life cycle of flowering plants. The present study found that SlMYB72 was highly expressed in the pollen and tapetum of tomato flowers. Downregulation of SlMYB72 led to a decrease in the amounts of seeds due to abnormal pollen development compared with wild-type plants. Downregulation of SlMYB72 delayed tapetum degradation and inhibited autophagy in tomato anther. Overexpression of SlMYB72 led to abnormal pollen development and delayed tapetum degradation. Expression levels of some autophagy-related genes (ATGs) were decreased in SlMYB72 downregulated plants and increased in overexpression plants. SlMYB72 was directly bound to ACCAAC/ACCAAA motif of the SlATG7 promoter and activated its expression. Downregulation of SlATG7 inhibited the autophagy process and tapetum degradation, resulting in abnormal pollen development in tomatoes. These results indicated SlMYB72 affects the tapetum degradation and pollen development by transcriptional activation of SlATG7 and autophagy in tomato anther. The study expands the understanding of the regulation of autophagy by SlMYB72, uncovers the critical role that autophagy plays in pollen development, and provides potential candidate genes for the production of male-sterility in plants.

Acknowledgements

This work was supported by the National Natural Science Foundation of China (32172596), the Technology Innovation and Application Development Project in Chongqing (cstc2021jscx-cylhX0115), the Chongqing Talents Innovation Leading Talents Project (cstc2022ycjh-bgzxm0018), the Tianfu Scholar Program of Sichuan Province (Department of Human Resources and Social Security of Sichuan Province 2021-58), and the Fundamental Research Funds for the Central Universities (2021CDJZYJH-002), and The Graduate Research and Innovation Foundation of Chongqing, China (CYB22048). We thank Jie Ye (Huazhong Agricultural University), Jianye Chen (South China Agricultural University), and Jianfei Kuang (South China Agricultural University) for providing experimental instruction.

Author contributions

W.D., M.W., H.L., and Z.L. designed the research. M.W., Q.Z., G.W., X.X., X.H., Z.G., and Y.C. carried out experiments and collected and analysed the data. W.D., M.W., H.L. wrote the manuscript.

Data availability statement

All relevant data and figures in this study can be found within the article and its supporting materials.

Conflict of interest

The authors declare that they have no conflicts of interest.

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