%A Xiaoxia LU, Weikun HU, Shengdao XIONG, Guopeng XU, Fen LAN %T Tim-3 mRNA expression in peripheral blood lymphocytes from asthmatic patients %0 Journal Article %D 2009 %J Front. Med. %J Frontiers of Medicine %@ 2095-0217 %R 10.1007/s11684-009-0033-6 %P 187-190 %V 3 %N 2 %U {https://journal.hep.com.cn/fmd/EN/10.1007/s11684-009-0033-6 %8 2009-06-05 %X
The study aimed to detect the expression of the Th1-specific cell surface protein T cell Ig and mucin domain-containing molecule-3 (Tim-3) mRNA in peripheral blood lymphocytes isolated from asthmatic patients and to examine the correlation among Tim-3 mRNA, interleukin-4 (IL-4), interferon-γ (IFN-γ) level, and FEV1/FVC (force expiratory volume in one second/forced vital capacity) to explore the role of Tim-3 in the development and progression of asthmatic inflammation. Tim-3 mRNA expression was detected by reverse transcription polymerase chain reaction (RT-PCR). The IL-4 and IFN-γ levels were determined by using enzyme-linked immunosorbent assay (ELISA). The correlation among Tim-3 mRNA, IL-4, IFN-γ level, and pulmonary ventilatory capacity was analyzed. The expression of Tim-3 mRNA in patients with acute asthma exacerbation was 0.39±0.06, significantly higher than that in patients at the remission stage and controls (0.18±0.05 and 0.07±0.03,