Design of tandem genes cluster for isoflavone
engineering

doi:10.1007/s11684-009-0057-y

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Frontiers of Medicine ›› 2009, Vol. 3 ›› Issue (3) : 292-296. DOI: 10.1007/s11684-009-0057-y

Research articles

Design of tandem genes cluster for isoflavone engineering

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Design of tandem genes cluster for isoflavone engineering

Xunli XIA PhD , Guangxiao YANG PhD , Guangyuan HE PhD ,

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Abstract

In this paper, a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported. A tandem genes cluster CHS-CHI-IFS (rIFS) of secondary metabolites of plant isoflavones was constructed by using the chalcone synthase (CHS), chalcone isomerase (CHI), and isoflavone synthase (IFS) (GenBank accession numbers EU526827, EU526829, EU526830) in only one recombination with the pET22b vector. The resulting expression vector pET-rIFS was heterogeneously expressed and co-incubated with barrenwort extractions, and the genistein-like component was detected.