Relationship of endothelial nitric oxide synthase gene polymorphism with blood pressure, lipid profile and blood glucose level

Relationship of endothelial nitric oxide synthase gene polymorphism with blood pressure, lipid profile and blood glucose level

Abstract

To study the relationship of the polymorphism of endothelial nitric oxide synthase (eNOS) gene and blood pressure, lipid profiles and blood glucose level. By using PCR-RFLP, the eNOS Glu298Asp gene polymorphism was detected in 184 patients with essential hypertension and 196 matched healthy individuals with normal blood pressure. Taking into account eNOS Glu298Asp polymorphisms, the relationship of blood pressure with triglycerides (TG), total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL) and blood glucose level was analyzed. The distribution of eNOS Glu298Asp polymorphism had no significant difference between different blood pressure groups and gender groups, but there was a significant difference between different age groups, diastolic blood pressure groups or BMI groups (P < 0.05). Asp/Asp genotype significantly increased the risk of hypertension in individuals with serum TC above 5.4 mmol/L (P = 0.03, OR = 2.65). eNOS Glu298Asp polymorphism and serum lipid could synergistically modulate the blood pressure. eNOS Asp/Asp genotype could significantly increase the risk of hypertension in individuals with serum TC over 5.4 mmol/L. eNOS Glu298Asp in combination with serum TC could be used to predict the risk of hypertension.

参考文献

1. Pereira A C Sposito A C Mota G F Cunha R S Herkenhoff F L Mill J G Krieger J E Endothelialnitric oxide synthase gene variant modulates the relationship betweenserum cholesterol levels and blood pressure in the general population:New evidence for a direct effect of lipids in arterial blood pressureAtherosclerosis 2006 184(1)193200. doi:10.1016/j.atherosclerosis.2005.03.035
2. Heltianu C Costache G Gafencu A Diaconu M Bodeanu M Cristea C Azibi K Poenaru L Simionescu M Relationshipof eNOS gene variants to diseases that have in common an endothelialcell dysfunctionJ Cell Mol Med 2005 9(1)135142. doi:10.1111/j.1582‐4934.2005.tb00343.x
3. Wolff B Grabe H J Schlüter C Popowski K Völzke H Lüdemann J John U Felix S B Cascorbi I Endothelialnitric oxide synthase Glu298Asp gene polymorphism, blood pressureand hypertension in a general population sampleJ Hypertens 2005 23(7)13611366. doi:10.1097/01.hjh.0000173518.06353.c2
4. Yoon S Shin C Park H Y Moon J Kim E Kim H T Min J Jo S A Jo I Endothelial nitric oxide synthase gene is associated withvesselClin Chim Acta 2005 353(1–2)177185. doi:10.1016/j.cccn.2004.10.018
5. Kishimoto T Misawa Y Kaetu A Nagai M Osaki Y Okamoto M Yoshida S Kurosawa Y Fukumoto S eNOS Glu298Asppolymorphism and hypertension in a cohort study in JapanesePrev Med 2004 39(5)927931. doi:10.1016/j.ypmed.2004.03.030
6. Zhu H Wang X Dong Y Treiber F A Snieder H Influence of the eNOS gene on developmentof blood pressure and left ventricular mass: longitudinal findingsin multiethnic youthPharmacogent Genomics 2005 15(9)669675