Frontiers in Biology >

2013 , Vol. 8 >Issue 6: 611 - 617

DOI: https://doi.org/10.1007/s11515-013-0282-2

RESEARCH ARTICLE

Optimization of fermentation medium for xylanase-producing strain Xw2

  • Bingying YE 1,2,3 ,
  • Ting XUE 1,2 ,
  • Shichao YE 1,2 ,
  • Shengyan XU 1,2 ,
  • Weiyan LI 1,2 ,
  • Jihua LU 1,2 ,
  • Fang WEI 4 ,
  • Wenjin HE 1,2 ,
  • Youqiang CHEN , 1,2,3
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  • 1. College of Life Sciences, Fujian Normal University, Fuzhou 350108, China
  • 2. Key Laboratory of Developmental and Neural, Fuzhou 350108, China
  • 3. Key Laboratory of Sugarcane Biology and Genetic Breeding of Ministry of Agriculture, Fuzhou 350108, China
  • 4. Environmental Protection Agency, Fuzhou, Sanming 350400, China

Received date: 02 Jul 2013

Accepted date: 10 Sep 2013

Published date: 01 Dec 2013

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg
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Abstract

To improve the fermentation yield of xylanase by optimizing the fermentation conditions for strain Xw2, a Plackett-Burman design was used to evaluate the effects of eight variables on xylanase production by strain Xw2. The steepest ascent (descent) method was used to approach the optimal response surface experimental area. The optimal fermentation conditions were obtained by central composite design and response surface analysis. The results showed that the composition of the optimal fermentation medium was corn cob+ 1.5% wheat bran (1:1), 0.04% MnSO4, 0.04% K2HPO4·3H2O, and an inoculum size of 6% in 50 mL liquid volume (pH= 6.0). The optimal culture conditions were 28°C at 150 r/min for 54.23 h. The results of this study can serve as the basis for the industrial production and application of xylanase.

Key words： enzyme activity; fermentation; Plackett-Burman test; xylan

Cite this article

Bingying YE , Ting XUE , Shichao YE , Shengyan XU , Weiyan LI , Jihua LU , Fang WEI , Wenjin HE , Youqiang CHEN . Optimization of fermentation medium for xylanase-producing strain Xw2[J]. Frontiers in Biology, 2013 , 8(6) : 611 -617 . DOI: 10.1007/s11515-013-0282-2

Acknowledegments

This work was supported by grants from the Key Laboratory of Sugarcane Biology and Genetic Breeding of Ministry of Agriculture and the foundation of the Construction of Modern Agricultural Technology System of China (ID: CARS-20-4-4).
Compliance with ethics guidelines

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